ArticleMicrobes and infection2012
Impact of the deletion of the six mce operons in Mycobacterium smegmatis.
Article in Microbes and infection, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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Who cites it
34 citing papers in PubMed, 61 citations in OpenAlex.
- Mce transport systems inJournal of bacteriology · 2026Review
- Comparative in silico analysis of mce operons across Mycobacteriaceae and Nocardiaceae: Insights into genetic arrangements and regulatory mechanisms.Archives of microbiology · 2025Article
- Steroid Hydroxylation by Mutant Cytochrome P450 BM3-LG23 Using Two Expression Chassis.International journal of molecular sciences · 2025Article
- Genetic determinants ofMicrobiology spectrum · 2025Article
- Article
- Effect of Host Cholesterol on the Membrane Dynamics of Outer Membrane Lipids of Mycobacteria.Chemistry, an Asian journal · 2023Article
- Immuno-reactivity evaluation of Mce-truncated subunit candidate vaccine against Mycobacterium avium subspecies paratuberculosis challenge in the goat models.BMC veterinary research · 2023Article
- Article
- Structure of an endogenous mycobacterial MCE lipid transporter.Research square · 2023Article
- Rational development of mycobacteria cell factory for advancing the steroid biomanufacturing.World journal of microbiology & biotechnology · 2022Review
- Role of Ring6 in the Function of the E. coli MCE Protein LetB.Journal of molecular biology · 2022Article
- Evidence for the Mycobacterial Mce4 Transporter Being a Multiprotein Complex.Journal of bacteriology · 2021Article
- Biofilms of the non-tuberculousCell surface (Amsterdam, Netherlands) · 2020Article
- Article
- Unraveling the 17β-Estradiol Degradation Pathway inFrontiers in microbiology · 2020Article
- Mammalian cell entry operons; novel and major subset candidates for diagnostics with special reference toThe veterinary quarterly · 2019Review
- Intermembrane transport: Glycerophospholipid homeostasis of the Gram-negative cell envelope.Proceedings of the National Academy of Sciences of the United States of America · 2019Review
- Review
- New insights into the genome of Rhodococcus ruber strain Chol-4.BMC genomics · 2019Article
- The extracellular matrix of mycobacterial biofilms: could we shorten the treatment of mycobacterial infections?Microbial cell (Graz, Austria) · 2019Review
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 3 countries.
Funding
Abstract
The Mycobacterium smegmatis genome contains six operons designated mce (mammalian cell entry). These operons, which encode membrane and exported proteins, are highly conserved in pathogenic and non-pathogenic mycobacteria. Although the function of the Mce protein family has not yet been established in Mycobacterium smegmatis, the requirement of the mce4 operon for cholesterol utilization and uptake by Mycobacterium tuberculosis has recently been demonstrated. In this study, we report the construction of an M. smegmatis knock-out mutant deficient in the expression of all six mce operons. The consequences of these mutations were studied by analyzing physiological parameters and phenotypic traits. Differences in colony morphology, biofilm formation and aggregation in liquid cultures were observed, indicating that mce operons of M. smegmatis are implicated in the maintenance of the surface properties of the cell. Importantly, the mutant strain showed reduced cholesterol uptake when compared to the parental strain. Further cholesterol uptake studies using single mce mutant strains showed that the mutation of operon mce4 was reponsible for the cholesterol uptake failure detected in the sextuple mce mutant. This finding demonstrates that mce4operon is involved in cholesterol transport in M. smegmatis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.