ArticleJournal of applied microbiology2012
Development and application of a polymicrobial, in vitro, wound biofilm model.
Article in Journal of applied microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 68 citations in OpenAlex.
- An update on biofilms in acute and chronic wounds: Incidence, clinical evidence, diagnosis, prevention, and treatment.Journal of internal medicine · 2026Review
- Preparing a Dual-SpeciesMolecular pharmaceutics · 2025Article
- Enhancing Pathogen Detection in Implant-Related Infections through Chemical Antibiofilm Strategies: A Comprehensive Review.Antibiotics (Basel, Switzerland) · 2024Review
- A Three-Dimensional Model of Bacterial Biofilms and Its Use in Antimicrobial Susceptibility Testing.Microorganisms · 2024Article
- Development of Martian saline seep models and their implications for planetary protection.Biofilm · 2023Article
- Quantitative Insights and Visualization of Antimicrobial Tolerance in Mixed-Species Biofilms.Biomedicines · 2023Article
- Evaluation of the polyphenolic profile of native Ecuadorian stingless bee honeys (Current research in food science · 2023Article
- 3D Biofilm Models Containing Multiple Species for Antimicrobial Testing of Wound Dressings.Microorganisms · 2022Article
- 3D spatial organization and improved antibiotic treatment of aFrontiers in microbiology · 2022Article
- Reversal of Polymicrobial Biofilm Tolerance to Ciprofloxacin by Blue Light plus Carvacrol.Microorganisms · 2021Article
- Pseudomonas aeruginosa reverse diauxie is a multidimensional, optimized, resource utilization strategy.Scientific reports · 2021Article
- Spatially resolved analysis of Pseudomonas aeruginosa biofilm proteomes measured by laser ablation sample transfer.PloS one · 2021Article
- The evolution from the two stage to the one stage procedure for biofilm based periprosthetic joint infections (PJI).Biofilm · 2020Article
- Article
- A multimodel regime for evaluating effectiveness of antimicrobial wound care products in microbial biofilms.Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society · 2020Article
- The Open Challenge ofFrontiers in bioengineering and biotechnology · 2020Review
- Quorum sensing-induced phenotypic switching as a regulatory nutritional stress response in a competitive two-species biofilm: An individual-based cellular automata model.Journal of biosciences · 2020Article
- Current Status of In Vitro Models and Assays for Susceptibility Testing for Wound Biofilm Infections.Biomedicines · 2019Review
- Virulence Factors ofThe Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2019Review
- Prevention of Staphylococcus aureus biofilm formation by antibiotics in 96-Microtiter Well Plates and Drip Flow Reactors: critical factors influencing outcomes.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
aimsThe goal of this investigation was to develop an in vitro, polymicrobial, wound biofilm capable of supporting the growth of bacteria with variable oxygen requirements. METHODS AND
resultsThe strict anaerobe Clostridium perfringens was isolated by cultivating wound homogenates using the drip-flow reactor (DFR), and a three-species biofilm model was established using methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa and Cl. perfringens in the colony-drip-flow reactor model. Plate counts revealed that MRSA, Ps. aeruginosa and Cl. perfringens grew to 7·39 ± 0·45, 10·22 ± 0·22 and 7·13 ± 0·77 log CFU per membrane, respectively. The three-species model was employed to evaluate the efficacy of two antimicrobial dressings, Curity™ AMD and Acticoat™, compared to sterile gauze controls. Microbial growth on Curity™ AMD and gauze was not significantly different, for any species, whereas Acticoat™ was found to significantly reduce growth for all three species.
conclusionsUsing the colony-DFR, a three-species biofilm was successfully grown, and the biofilms displayed a unique structure consisting of distinct layers that appeared to be inhabited exclusively or predominantly by a single species. SIGNIFICANCE AND IMPACT OF THE STUDY: The primary accomplishment of this study was the isolation and growth of an obligate anaerobe in an in vitro model without establishing an artificially anaerobic environment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.