ArticleJournal of biomedical optics2012
Phasor imaging with a widefield photon-counting detector.
Article in Journal of biomedical optics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Deep Learning for Fluorescence Lifetime Predictions Enables High-Throughput In Vivo Imaging.Journal of the American Chemical Society · 2025Article
- Applying first & second harmonic spectral phasor analysis on a single-wavelength calcium fluorophore.Biochemistry and biophysics reports · 2025Article
- Towards measurements of absolute membrane potential in Bacillus subtilis using fluorescence lifetime.bioRxiv : the preprint server for biology · 2024Article
- Label-free fluorescence lifetime imaging for the assessment of cell viability in living tumor fragments.Journal of biomedical optics · 2024Article
- Multiplexed near infrared fluorescence lifetime imaging in turbid media.Journal of biomedical optics · 2024Article
- Article
- Fluorescence lifetime imaging with a megapixel SPAD camera and neural network lifetime estimation.Scientific reports · 2020Article
- Direct Photon-by-Photon Analysis of Time-Resolved Pulsed Excitation Data using Bayesian Nonparametrics.Cell reports. Physical science · 2020Article
- Wide-field time-gated SPAD imager for phasor-based FLIM applications.Methods and applications in fluorescence · 2020Article
- In vitro and in vivo phasor analysis of stoichiometry and pharmacokinetics using short-lifetime near-infrared dyes and time-gated imaging.Journal of biophotonics · 2019Article
- Phasor-based widefield FLIM using a gated 512×512 single-photon SPAD imager.Proceedings of SPIE--the International Society for Optical Engineering · 2019Article
- Fluorescence lifetime imaging with a single-photon SPAD array using long overlapping gates: an experimental and theoretical study.Proceedings of SPIE--the International Society for Optical Engineering · 2019Article
- AlliGator: A Phasor Computational Platform for FastOptical molecular probes, imaging and drug delivery · 2017Article
- Design Rules for Membrane-Embedded Voltage-Sensing Nanoparticles.Biophysical journal · 2017Article
- Article
- A method to quantify FRET stoichiometry with phasor plot analysis and acceptor lifetime ingrowth.Biophysical journal · 2015Article
- Architecture and applications of a high resolution gated SPAD image sensor.Optics express · 2014Article
- Parallel excitation-emission multiplexed fluorescence lifetime confocal microscopy for live cell imaging.Optics express · 2014Article
- Development of new photon-counting detectors for single-molecule fluorescence microscopy.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Review
- Application of FRET probes in the analysis of neuronal plasticity.Frontiers in neural circuits · 2013Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Fluorescence lifetime can be used as a contrast mechanism to distinguish fluorophores for localization or tracking, for studying molecular interactions, binding, assembly, and aggregation, or for observing conformational changes via Förster resonance energy transfer (FRET) between donor and acceptor molecules. Fluorescence lifetime imaging microscopy (FLIM) is thus a powerful technique but its widespread use has been hampered by demanding hardware and software requirements. FLIM data is often analyzed in terms of multicomponent fluorescence lifetime decays, which requires large signals for a good signal-to-noise ratio. This confines the approach to very low frame rates and limits the number of frames which can be acquired before bleaching the sample. Recently, a computationally efficient and intuitive graphical representation, the phasor approach, has been proposed as an alternative method for FLIM data analysis at the ensemble and single-molecule level. In this article, we illustrate the advantages of combining phasor analysis with a widefield time-resolved single photon-counting detector (the H33D detector) for FLIM applications. In particular we show that phasor analysis allows real-time subsecond identification of species by their lifetimes and rapid representation of their spatial distribution, thanks to the parallel acquisition of FLIM information over a wide field of view by the H33D detector. We also discuss possible improvements of the H33D detector's performance made possible by the simplicity of phasor analysis and its relaxed timing accuracy requirements compared to standard time-correlated single-photon counting (TCSPC) methods.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.