Evidence map›Paper›PMID 22352645›Full record

ArticleJournal of biomedical optics2012

High-throughput optofluidic system for the laser microsurgery of oocytes.

Charlie Chandsawangbhuwana, Linda Z Shi, Qingyuan Zhu, Mark C Alliegro, Michael W Berns

Abstract read
In one paragraph

Article in Journal of biomedical optics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Optofluidic cell manipulation for a biological microbeam.The Review of scientific instruments · 2013
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Charlie ChandsawangbhuwanaUniversity of California, San Diego, Department of Bioengineering, 9500 Gilman Drive, La Jolla, California 92093, USA. cchandsa@ucsd.edu
Linda Z Shi
Qingyuan Zhu
Mark C Alliegro
Michael W Berns

Funding

Rediscovery of the NucleolinusR01GM088503 · NIGMS · MARINE BIOLOGICAL LABORATORY · PI ALLIEGRO, MARK COLLIN · 2009 to 2012
$1.2M
NIGMS NIH HHS GM088503NIGMS NIH HHS R01 GM088503
6 · The paper itself

Abstract

This study combines microfluidics with optical microablation in a microscopy system that allows for high-throughput manipulation of oocytes, automated media exchange, and long-term oocyte observation. The microfluidic component of the system transports oocytes from an inlet port into multiple flow channels. Within each channel, oocytes are confined against a microfluidic barrier using a steady fluid flow provided by an external computer-controlled syringe pump. This allows for easy media replacement without disturbing the oocyte location. The microfluidic and optical-laser microbeam ablation capabilities of the system were validated using surf clam (Spisula solidissima) oocytes that were immobilized in order to permit ablation of the 5 μm diameter nucleolinus within the oocyte nucleolus. Oocytes were the followed and assayed for polar body ejection.

Indexed as

AnimalsCell NucleusEquipment DesignHigh-Throughput Screening AssaysLaser TherapyMicrofluidic Analytical TechniquesMicrosurgeryOocytesOptics and PhotonicsReproducibility of ResultsSpisula

Identifiers

PMID22352645
PMCPMC3380719

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.