ArticleBioconjugate chemistry2012
Dual 19F/1H MR gene reporter molecules for in vivo detection of β-galactosidase.
Article in Bioconjugate chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 52 citations in OpenAlex.
- Nanoparticle ultrastructure allows reversible pH sensitivity usingNanoscale advances · 2026Article
- The role of responsive MRI probes in the past and the future of molecular imaging.Chemical science · 2024Review
- A Genetically Engineered Reporter System Designed forJournal of the American Chemical Society · 2024Article
- Enzyme-activatable fluorescent probes for β-galactosidase: from design to biological applications.Chemical science · 2021Review
- Article
- Recent progress in the imaging detection of enzyme activitiesRSC advances · 2019Review
- Fluorinated Paramagnetic Complexes: Sensitive and Responsive Probes for Magnetic Resonance Spectroscopy and Imaging.Frontiers in chemistry · 2018Review
- MRI Reporter Genes for Noninvasive Molecular Imaging.Molecules (Basel, Switzerland) · 2016Review
- Development of Timd2 as a reporter gene for MRI.Magnetic resonance in medicine · 2016Article
- Advances in using MRI probes and sensors for in vivo cell tracking as applied to regenerative medicine.Disease models & mechanisms · 2015Review
- A fluorogenic probe for β-galactosidase activity imaging in living cells.Molecular bioSystems · 2013Article
- Divalent metal transporter, DMT1: a novel MRI reporter protein.Magnetic resonance in medicine · 2013Article
- Novel S-Gal(®) analogs as (1)H MRI reporters for in vivo detection of β-galactosidase.Magnetic resonance imaging · 2013Article
- MRI reporter genes: applications for imaging of cell survival, proliferation, migration and differentiation.NMR in biomedicine · 2013Review
- New frontiers and developing applications in 19F NMR.Progress in nuclear magnetic resonance spectroscopy · 2013Review
- Dual Expression of Magnetosome-Associated GenesMolecular imagingArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Increased emphasis on personalized medicine and novel therapies requires the development of noninvasive strategies for assessing biochemistry in vivo. The detection of enzyme activity and gene expression in vivo is potentially important for the characterization of diseases and gene therapy. Magnetic resonance imaging (MRI) is a particularly promising tool, since it is noninvasive and has no associated radioactivity, yet penetrates deep tissue. We now demonstrate a novel class of dual (1)H/(19)F nuclear magnetic resonance (NMR) lacZ gene reporter molecule to specifically reveal enzyme activity in human tumor xenografts growing in mice. We report the design, synthesis, and characterization of six novel molecules and evaluation of the most effective reporter in mice in vivo. Substrates show a single (19)F NMR signal and exposure to β-galactosidase induces a large (19)F NMR chemical shift response. In the presence of ferric ions, the liberated aglycone generates intense proton MRI T(2) contrast. The dual modality approach allows both the detection of substrate and the imaging of product enhancing the confidence in enzyme detection.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.