ArticleThe Journal of biological chemistry2012
Characterization of DNA primase complex isolated from the archaeon, Thermococcus kodakaraensis.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- Archaeal family B DNA polymerase facilitates lagging strand DNA replication in the Thermococcales.Nucleic acids research · 2026Article
- The monomeric archaeal primase from Nanoarchaeum equitans harbours the features of heterodimeric archaeoeukaryotic primases and primes sequence-specifically.Nucleic acids research · 2023Article
- Review
- Pol B, a Family B DNA Polymerase, in Thermococcus kodakarensis is Important for DNA Repair, but not DNA Replication.Microbes and environments · 2019Article
- Mimivirus encodes a multifunctional primase with DNA/RNA polymerase, terminal transferase and translesion synthesis activities.Nucleic acids research · 2019Article
- Divalent Cations Alter the Rate-Limiting Step of PrimPol-Catalyzed DNA Elongation.Journal of molecular biology · 2019Article
- Novel Families of Archaeo-Eukaryotic Primases Associated with Mobile Genetic Elements of Bacteria and Archaea.Journal of molecular biology · 2018Article
- The GAN Exonuclease or the Flap Endonuclease Fen1 and RNase HII Are Necessary for Viability of Thermococcus kodakarensis.Journal of bacteriology · 2017Article
- TruePrime is a novel method for whole-genome amplification from single cells based on TthPrimPol.Nature communications · 2016Article
- Primase-polymerases are a functionally diverse superfamily of replication and repair enzymes.Nucleic acids research · 2015Review
- A primase subunit essential for efficient primer synthesis by an archaeal eukaryotic-type primase.Nature communications · 2015Article
- The place of RNA in the origin and early evolution of the genetic machinery.Life (Basel, Switzerland) · 2014Article
- A novel mechanism for regulating the activity of proliferating cell nuclear antigen by a small protein.Nucleic acids research · 2014Article
- A highly divergent archaeo-eukaryotic primase from the Thermococcus nautilus plasmid, pTN2.Nucleic acids research · 2014Article
- PrimPol, an archaic primase/polymerase operating in human cells.Molecular cell · 2013Article
- RecJ-like protein from Pyrococcus furiosus has 3'-5' exonuclease activity on RNA: implications for proofreading of 3'-mismatched RNA primers in DNA replication.Nucleic acids research · 2013Article
- Replicative DNA polymerases.Cold Spring Harbor perspectives in biology · 2013Review
- Thermococcus kodakarensis has two functional PCNA homologs but only one is required for viability.Extremophiles : life under extreme conditions · 2013Article
- Establishing the human rolling circle reaction.Cell cycle (Georgetown, Tex.) · 2012Article
- Formation of dAMP-glycerol and dAMP-Tris derivatives by Thermococcus kodakaraensis DNA primase.The Journal of biological chemistry · 2012Article
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
In most organisms, DNA replication is initiated by DNA primases, which synthesize primers that are elongated by DNA polymerases. In this study, we describe the isolation and biochemical characterization of the DNA primase complex and its subunits from the archaeon Thermococcus kodakaraensis. The T. kodakaraensis DNA primase complex is a heterodimer containing stoichiometric levels of the p41 and p46 subunits. The catalytic activity of the complex resides within the p41 subunit. We show that the complex supports both DNA and RNA synthesis, whereas the p41 subunit alone marginally produces RNA and synthesizes DNA chains that are longer than those formed by the complex. We report that the T. kodakaraensis primase complex preferentially interacts with dNTP rather than ribonucleoside triphosphates and initiates RNA as well as DNA chains de novo. The latter findings indicate that the archaeal primase complex, in contrast to the eukaryote homolog, can initiate DNA chain synthesis in the absence of ribonucleoside triphosphates. DNA primers formed by the archaeal complex can be elongated extensively by the T. kodakaraensis DNA polymerase (Pol) B, whereas DNA primers formed by the p41 catalytic subunit alone were not. Supplementation of reactions containing the p41 subunit with the p46 subunit leads to PolB-catalyzed DNA synthesis. We also established a rolling circle reaction using a primed 200-nucleotide circle as the substrate. In the presence of the T. kodakaraensis minichromosome maintenance (MCM) 3' → 5' DNA helicase, PolB, replication factor C, and proliferating cell nuclear antigen, long leading strands (>10 kb) are produced. Supplementation of such reactions with the DNA primase complex supported lagging strand formation as well.
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