ArticleCroatian medical journal2012
Highly efficient nuclear DNA typing of the World War II skeletal remains using three new autosomal short tandem repeat amplification kits with the extended European Standard Set of loci.
Article in Croatian medical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Review
- Article
- DNA degradation in human teeth exposed to thermal stress.Scientific reports · 2021Article
- DNA recovery and analysis from skeletal material in modern forensic contexts.Forensic sciences research · 2019Review
- As solid as a rock-comparison of CE- and MPS-based analyses of the petrosal bone as a source of DNA for forensic identification of challenging cranial bones.International journal of legal medicine · 2018Article
- Identification of human remains from the Second World War mass graves uncovered in Bosnia and Herzegovina.Croatian medical journal · 2015Article
- Slovenian population data for five new European Standard Set short tandem repeat loci and SE33 locus.Croatian medical journal · 2014Article
- High efficiency protocol of DNA extraction fromActa theriologica · 2014Article
- STR-typing of ancient skeletal remains: which multiplex-PCR kit is the best?Croatian medical journal · 2012Article
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5 authors.
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Abstract
aimTo perform an efficiency study of three new amplification kits with the extended European Standard Set (ESS) of loci for autosomal short tandem repeat (STR) typing of skeletal remains excavated from the World War II mass graves in Slovenia.
methodsIn the beginning of the 2011, we analyzed 102 bones and teeth using the PowerPlex ESX 17 System (Promega), AmpFiSTR NGM PCR Amplification Kit (Applied Biosystems), and Investigator ESSplex Kit (Qiagen). We cleaned the bones and teeth, removed surface contamination, and ground them into a powder using liquid nitrogen. Prior to DNA isolation with Biorobot EZ1 (Qiagen), 0.5 g bone or tooth powder was decalcified. Nuclear DNA of the samples was quantified using real-time polymerase chain reaction. All three kits used the same extract with the amplification conditions recommended by the manufacturers.
resultsWe extracted up to 131 ng DNA/g of powder from the bones and teeth. All three amplification kits showed very similar efficiency, since DNA typing was successful with all amplification kits in 101 out of 102 bones and teeth, which represents a 99% success rate.
conclusionThe commercially available ESX 17, ESSplex, and NGM kits are highly reliable for STR typing of World War II skeletal remains with the DNA extraction method optimized in our laboratory.
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