ArticleAngiogenesis2012
Angiogenic properties of myofibroblasts isolated from normal human skin wounds.
Article in Angiogenesis, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Integrative bioinformatic analysis prioritizes TIMP1 and FN1 as angiogenesis-related candidate genes in diabetic foot ulcers.PloS one · 2026Article
- Endothelial Effects in the Elderly: Fibroblast Regulation in Soft Tissue Healing.Journal of cellular physiology · 2025Article
- Polyethylene glycol-polyester based temperature-sensitive hydrogel delivering mesenchymal stem cell-derived exosomes enhances acute skin wound healing.Frontiers in bioengineering and biotechnology · 2025Article
- Isolation and Culture of Human Dermal Microvascular Endothelial Cells.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Microvesicles derived from dermal myofibroblasts modify the integrity of the blood and lymphatic barriers using distinct endocytosis pathways.Journal of extracellular biology · 2024Article
- Decellularized heart extracellular matrix alleviates activation of hiPSC-derived cardiac fibroblasts.Bioactive materials · 2024Article
- The diffusion of normal skin wound myofibroblast-derived microvesicles differs according to matrix composition.Journal of extracellular biology · 2024Article
- Cerium oxide nanoparticles: Synthesis methods and applications in wound healing.Materials today. Bio · 2023Review
- VEGF-Encoding, Gene-Activated Collagen-Based Matrices Promote Blood Vessel Formation and Improved Wound Repair.ACS applied materials & interfaces · 2023Article
- Adult skin fibroblast state change in murine wound healing.Scientific reports · 2023Article
- Tissue Engineering in Gynecology.International journal of molecular sciences · 2022Review
- Evaluating polymeric biomaterials to improve next generation wound dressing design.Biomaterials research · 2022Review
- Novel insights into negative pressure wound healing from an in situ porcine perspective.Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society · 2022Article
- Abdominoplasty Skin-Based Dressing for Deep Wound Treatment-Evaluation of Different Methods of Preparation on Therapeutic Potential.Pharmaceutics · 2021Article
- Cloves (Syzygium aromaticum) fluid gel on healing of pododermatitis in rabbits.Veterinary research communications · 2021Article
- Curcumin Suppresses TGF-β1-Induced Myofibroblast Differentiation and Attenuates Angiogenic Activity of Orbital Fibroblasts.International journal of molecular sciences · 2021Article
- In Vitro Prevascularization of Self-Assembled Human Bone-Like Tissues and Preclinical Assessment Using a Rat Calvarial Bone Defect Model.Materials (Basel, Switzerland) · 2021Article
- Simultaneously targeting cancer-associated fibroblasts and angiogenic vessel as a treatment for TNBC.The Journal of experimental medicine · 2021Article
- Conditioned medium produced by fibroblasts cultured in low oxygen pressure allows the formation of highly structured capillary-like networks in fibrin gels.Scientific reports · 2020Article
- Cellular Interactions in the Intestinal Stem Cell Niche.Archivum immunologiae et therapiae experimentalis · 2019Review
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7 authors.
Funding
Abstract
During wound healing, angiogenesis plays a crucial role in inducing adequate perfusion of the new tissue, thereby allowing its survival. This angiogenic process contributes to the formation of granulation tissue, alongside myofibroblasts. Myofibroblasts are cells specialized in wound contraction and synthesis of new extracellular matrix. Fibroblasts, considered by some to be at the origin of myofibroblasts, have already been shown to promote neovascularization. Thus, we hypothesized that myofibroblasts play a key role during angiogenic development in wound healing. We isolated myofibroblasts from normal human skin wounds and dermal microvascular endothelial cells (HDMVEC) and fibroblasts from skin. Using an in vitro fibrin-based model, we compared the proangiogenic activity of wound myofibroblasts to that of fibroblasts in the presence of HDMVEC. By immunostaining with collagen IV antibodies, we observed the formation of a capillary network significantly more developed when HDMVEC were cultured with myofibroblasts compared to the network formed in the presence of fibroblasts. The differences between these cell types did not result from a differential secretion of Vascular Endothelial Growth Factor or basic Fibroblast Growth Factor. However, in the presence of myofibroblasts, a significant decrease in matrix metalloproteinase activity was observed. This finding was correlated with a significant increase in Tissue Inhibitor of MetalloProteinase (TIMP)-1 and TIMP-3. Furthermore, inhibition of TIMP-1 secretion using shRNA significantly decreased myofibroblasts induced angiogenesis. These results led to the hypothesis that normal wound myofibroblasts contribute to the vascular network development during wound healing. Our data emphasize the critical role of wound myofibroblasts during healing.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.