ArticleProtoplasma2013
Determination of ACC-induced cell-programmed death in roots of Vicia faba ssp. minor seedlings by acridine orange and ethidium bromide staining.
Article in Protoplasma, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 76 citations in OpenAlex.
- Article
- In vitro anticancer potential of laminarin and fucoidan from Brown seaweeds.Scientific reports · 2023Article
- Hormonal crosstalk controls cell death induced by kinetin in roots of Vicia faba ssp. minor seedlings.Scientific reports · 2023Article
- (±)-3-Deoxyradicinin Induces Stomata Opening and Chloroplast Oxidative Stress in Tomato (International journal of molecular sciences · 2023Article
- Kinetin induces microtubular breakdown, cell cycle arrest and programmed cell death in tobacco BY-2 cells.Protoplasma · 2023Article
- Mechanism of kinetin-induced death of Vicia faba ssp. minor root cortex cells.Scientific reports · 2021Article
- Short-term cell death in tissues of Pulsatilla vernalis seeds from natural and ex situ conserved populations.Scientific reports · 2021Article
- Melatonin Application Modifies Antioxidant Defense and Induces Endoreplication in Maize Seeds Exposed to Chilling Stress.International journal of molecular sciences · 2021Article
- Article
- Cellular Base of Mint Allelopathy: Menthone Affects Plant Microtubules.Frontiers in plant science · 2020Article
- Apoptotic and DNA-damaging effects of yttria-stabilized zirconia nanoparticles on human skin epithelial cells.International journal of nanomedicine · 2019Article
- Proteolytic activities in cortex of apical parts of Vicia faba ssp. minor seedling roots during kinetin-induced programmed cell death.Protoplasma · 2017Article
- Membrane-related hallmarks of kinetin-induced PCD of root cortex cells.Plant cell reports · 2017Article
- The relationship between vacuolation and initiation of PCD in rice (Oryza sativa) aleurone cells.Scientific reports · 2017Article
- Melatonin Protects Cultured Tobacco Cells against Lead-Induced Cell DeathFrontiers in plant science · 2017Article
- Ethylacetate extract from Tetrastigma hemsleyanum induces apoptosis via the mitochondrial caspase-dependent intrinsic pathway in HepG2 cells.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- Oxidative damage and cell-programmed death induced in Zea mays L. by allelochemical stress.Ecotoxicology (London, England) · 2015Article
- Article
- Antitumor effects of a dual cancer-specific oncolytic adenovirus on colorectal cancerExperimental and therapeutic medicine · 2015Article
- Tissue Specific Promoters in Colorectal Cancer.Disease markers · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescence staining with acridine orange (AO) and ethidium bromide (EB) showed that nuclei of cortex root cells of 1-aminocyclopropane-1-carboxylic acid (ACC)-treated Vicia faba ssp. minor seedlings differed in color. Measurement of resultant fluorescence intensity (RFI) showed that it increased when the color of nuclear chromatin was changed from green to red, indicating that EB moved to the nuclei via the cell membrane which lost its integrity and stained nuclei red. AO/EB staining showed that changes in color of the nuclear chromatin were accompanied by DNA condensation, nuclei fragmentation, and chromatin degradation which were also shown after 4,6-diamidino-2-phenylindol staining. These results indicate that ACC induced programmed cell death. The increasing values of RFI together with the corresponding morphological changes of nuclear chromatin were the basis to prepare the standard curve; cells with green unchanged nuclear chromatin were alive while those with dark orange and bright red nuclei were dead. The cells with nuclei with green-yellow, yellow-orange, and bright orange chromatin with or without their condensation and fragmentation chromatin were dying. The prepared curve has became the basis to draw up the digital method for detection and determination of the number of living, dying, and dead cells in an in planta system and revealed that ACC induced death in about 20% of root cortex cells. This process was accompanied by increase in ion leakage, shortening of cells and whole roots, as well as by increase in weight and width of the apical part of roots and appearance of few aerenchymatic spaces while not by internucleosomal DNA degradation.
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