ArticlePflugers Archiv : European journal of physiology2012
Functional M3 cholinoreceptors are present in pacemaker and working myocardium of murine heart.
Article in Pflugers Archiv : European journal of physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 25 citations in OpenAlex.
- Treating heart failure by targeting the vagus nerve.Heart failure reviews · 2024Review
- The role of M3 receptors in regulation of electrical activity deteriorates in the rat heart during ageing.Current research in physiology · 2022Article
- GABA in developing rat skeletal muscle and motor neurons.Protoplasma · 2020Article
- Extracellular ATP and β-NAD alter electrical properties and cholinergic effects in the rat heart in age-specific manner.Purinergic signalling · 2019Article
- Choline Attenuates Cardiac Fibrosis by Inhibiting p38MAPK Signaling Possibly by Acting on MFrontiers in pharmacology · 2019Article
- Electrophysiological differences in cholinergic signaling between the hearts of summer and winter frogs (Rana temporaria).Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2018Article
- Atropine augments cardiac contractility by inhibiting cAMP-specific phosphodiesterase type 4.Scientific reports · 2017Article
- M3 cholinoreceptors alter electrical activity of rat left atrium via suppression of L-type CaJournal of physiology and biochemistry · 2017Article
- Acetylcholinesterase Inhibitors and Drugs Acting on Muscarinic Receptors- Potential Crosstalk of Cholinergic Mechanisms During Pharmacological Treatment.Current neuropharmacology · 2017Review
- Vagal nerve stimulation protects cardiac injury by attenuating mitochondrial dysfunction in a murine burn injury model.Journal of cellular and molecular medicine · 2013Article
- Effects of acetylcholine and noradrenalin on action potentials of isolated rabbit sinoatrial and atrial myocytes.Frontiers in physiology · 2012Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The presence of M3 cholinoreceptors and their role in mediation of action potential waveform modulation were determined by immunolabeling of receptor proteins and standard microelectrode technique, respectively. The sinoatrial node (SAN), which was determined as a connexin 43 negative area within the intercaval region, the surrounding atrial tissue, and the working ventricular myocardium exhibited labeling of both M3 and M2 receptors. However, the density of M3 and M2 labeling was about twofold higher in the SAN compared to working myocardium. The stimulation of M3 receptors was obtained by application of nonselective M1 and M3 muscarinic agonist pilocarpine (10(-5) M) in the presence of selective M2 blocker methoctramine (10(-7) M). Stimulation of M3 receptors provoked marked shortening of action potential duration in atrial and ventricular working myocardium. In the SAN, M3 stimulation leads to a significant reduction of sinus rhythm rate accompanied with slowing of diastolic depolarization and increase of action potential upstroke velocity. All electrophysiological effects of selective M3 stimulation were suppressed by specific blocker of M3 receptors 4-DAMP (10(-8) M). We conclude that M3 cholinoreceptors are present in pacemaker and working myocardium of murine heart, where they mediate negative cholinergic effects: slowing of sinus rhythm and shortening of action potentials.
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