ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2012
A knockout mutation in the lignin biosynthesis gene CCR1 explains a major QTL for acid detergent lignin content in Brassica napus seeds.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 71 citations in OpenAlex.
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- Homoeologous exchanges contribute to branch angle variations in rapeseed: Insights from transcriptome, QTL-seq and gene functional analysis.Plant biotechnology journal · 2024Article
- Identification of a cinnamoyl-CoA reductase from Cinnamomum cassia involved in trans-cinnamaldehyde biosynthesis.Planta · 2024Article
- A systems genomics and genetics approach to identify the genetic regulatory network for lignin content inFrontiers in plant science · 2024Article
- Genetic Dissection and Germplasm Selection of the Low Crude Fiber Component inFoods (Basel, Switzerland) · 2023Article
- The Flavonoid Biosynthesis and Regulation inInternational journal of molecular sciences · 2022Review
- A major yellow-seed QTL on chromosome A09 significantly increases the oil content and reduces the fiber content of seed in Brassica napus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Multi-omics analysis dissects the genetic architecture of seed coat content in Brassica napus.Genome biology · 2022Article
- QTL Identification for Stem Fiber, Strength and Rot Resistance in a DH Population from an Alien Introgression ofPlants (Basel, Switzerland) · 2022Article
- Incipient diploidization of the medicinal plant Perilla within 10,000 years.Nature communications · 2021Article
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- Long-read sequencing reveals widespread intragenic structural variants in a recent allopolyploid crop plant.Plant biotechnology journal · 2021Article
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- Extensive changes in gene expression and alternative splicing due to homoeologous exchange in rice segmental allopolyploids.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- Stable and novel QTL identification and new insights into the genetic networks affecting seed fiber traits in Brassica napus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- Transcriptomic comparison between developing seeds of yellow- and black-seeded Brassica napus reveals that genes influence seed quality.BMC plant biology · 2019Article
- Correlation Analysis of Phenolic Contents and Antioxidation in Yellow- and Black-SeededMolecules (Basel, Switzerland) · 2018Article
- Mapping of homoeologous chromosome exchanges influencing quantitative trait variation in Brassica napus.Plant biotechnology journal · 2017Article
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Seed coat phenolic compounds represent important antinutritive fibre components that cause a considerable reduction in value of seed meals from oilseed rape (Brassica napus). The nutritionally most important fibre compound is acid detergent lignin (ADL), to which a significant contribution is made by phenylpropanoid-derived lignin precursors. In this study, we used bulked-segregant analysis in a population of recombinant inbred lines (RILs) from a cross of the Chinese oilseed rape lines GH06 (yellow seed, low ADL) and P174 (black seed, high ADL) to identify markers with tight linkage to a major quantitative trait locus (QTL) for seed ADL content. Fine mapping of the QTL was performed in a backcross population comprising 872 BC(1)F(2) plants from a cross of an F(7) RIL from the above-mentioned population, which was heterozygous for this major QTL and P174. A 3:1 phenotypic segregation for seed ADL content indicated that a single, dominant, major locus causes a substantial reduction in ADL. This locus was successively narrowed to 0.75 cM using in silico markers derived from a homologous Brassica rapa sequence contig spanning the QTL. Subsequently, we located a B. rapa orthologue of the key lignin biosynthesis gene CINNAMOYL CO-A REDUCTASE 1 (CCR1) only 600 kbp (0.75 cM) upstream of the nearest linked marker. Sequencing of PCR amplicons, covering the full-length coding sequences of Bna.CCR1 homologues, revealed a locus in P174 whose sequence corresponds to the Brassica oleracea wild-type allele from chromosome C8. In GH06, however, this allele is replaced by a homologue derived from chromosome A9 that contains a loss-of-function frameshift mutation in exon 1. Genetic and physical map data infer that this loss-of-function allele has replaced a functional Bna.CCR1 locus on chromosome C8 in GH06 by homoeologous non-reciprocal translocation.
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