ArticlePlanta2012
Characterization, high-resolution mapping and differential expression of three homologous PAL genes in Coffea canephora Pierre (Rubiaceae).
Article in Planta, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 69 citations in OpenAlex.
- Molecular marker applications and functional genomics of drought tolerance in Coffea canephora: a comprehensive review.Molecular biology reports · 2026Review
- Prospects for improvement of coffee quality and stress resilience using genomic and genetic resources.Frontiers in plant science · 2026Review
- Article
- Pathway Gene Paralogues and Transcription Factors Differentially Associate with Contents of Picrosides in Tissues and Populations of a Medicinal Herb, Picrorhiza kurroa.Biochemical genetics · 2025Article
- Transcriptome analysis reveals the expression of genes involved in the formation of petal variegation in Impatiens morsei.Scientific reports · 2025Article
- Identification and expression analysis ofFrontiers in plant science · 2025Article
- Comparative analysis of the PAL gene family in nine citruses provides new insights into the stress resistance mechanism of Citrus species.BMC genomics · 2024Article
- Isolation and Characterization ofGenes · 2024Article
- Article
- Insights to Phenylalanine Ammonia Lyase (PAL) and Secondary Metabolism in Orchids: An in silico Approach.Biochemical genetics · 2024Article
- Transcriptome Analysis of Embryogenic and Non-Embryogenic Callus ofCurrent issues in molecular biology · 2023Article
- A draft genome of the medicinal plant Cremastra appendiculata (D. Don) provides insights into the colchicine biosynthetic pathway.Communications biology · 2022Article
- Phylogenomic analysis of phenylalanine ammonia-lyase (PAL) multigene family and their differential expression analysis in wheat (Frontiers in plant science · 2022Article
- Complexity of gene paralogues resolved in biosynthetic pathway of hepatoprotective iridoid glycosides in a medicinal herb, Picrorhiza kurroa through differential NGS transcriptomes.Molecular genetics and genomics : MGG · 2021Article
- Article
- Transcriptome profiling unveils the mechanism of phenylpropane biosynthesis in rhizome development of Caucasian clover.PloS one · 2021Article
- Metabolic Engineering of Isoflavones: An Updated Overview.Frontiers in plant science · 2021Review
- Transcriptomic Analysis ofInternational journal of molecular sciences · 2020Article
- Wheat straw increases the defense response and resistance of watermelon monoculture to Fusarium wilt.BMC plant biology · 2019Article
- Development and evaluation of a genome-wide Coffee 8.5K SNP array and its application for high-density genetic mapping and for investigating the origin of Coffea arabica L.Plant biotechnology journal · 2019Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phenylalanine ammonia lyase (PAL) is the first entry enzyme of the phenylpropanoid pathway producing phenolics, widespread constituents of plant foods and beverages, including chlorogenic acids, polyphenols found at remarkably high levels in the coffee bean and long recognized as powerful antioxidants. To date, whereas PAL is generally encoded by a small gene family, only one gene has been characterized in Coffea canephora (CcPAL1), an economically important species of cultivated coffee. In this study, a molecular- and bioinformatic-based search for CcPAL1 paralogues resulted successfully in identifying two additional genes, CcPAL2 and CcPAL3, presenting similar genomic structures and encoding proteins with close sequences. Genetic mapping helped position each gene in three different coffee linkage groups, CcPAL2 in particular, located in a coffee genome linkage group (F) which is syntenic to a region of Tomato Chromosome 9 containing a PAL gene. These results, combined with a phylogenetic study, strongly suggest that CcPAL2 may be the ancestral gene of C. canephora. A quantitative gene expression analysis was also conducted in coffee tissues, showing that all genes are transcriptionally active, but they present distinct expression levels and patterns. We discovered that CcPAL2 transcripts appeared predominantly in flower, fruit pericarp and vegetative/lignifying tissues like roots and branches, whereas CcPAL1 and CcPAL3 were highly expressed in immature fruit. This is the first comprehensive study dedicated to PAL gene family characterization in coffee, allowing us to advance functional studies which are indispensable to learning to decipher what role this family plays in channeling the metabolism of coffee phenylpropanoids.
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