ArticleMicrovascular research2012
Quantification of the endothelial surface glycocalyx on rat and mouse blood vessels.
Article in Microvascular research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
45 citing papers in PubMed, 85 citations in OpenAlex.
- Evaluating the endothelial glycocalyx from bench to bedside: a translational framework for microvascular assessment.Angiogenesis · 2026Review
- Tumor Metastasis in the Microcirculation.Advances in experimental medicine and biology · 2026Review
- Endothelial Surface Glycocalyx in Vascular Functions and Diseases.Advances in experimental medicine and biology · 2026Review
- Article
- Endothelial glycocalyx in different flow regions of the trabecular outflow pathway in bovine eyes.Frontiers in cell and developmental biology · 2025Article
- Microvascular Endothelial Glycocalyx Surface Layer Visualization and Quantification.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Factors influencing glycocalyx degradation: a narrative review.Frontiers in immunology · 2024Review
- Endothelial Glycocalyx in Aging and Age-related Diseases.Aging and disease · 2023Review
- Advances in the Physiology of Transvascular Exchange and A New Look At Rational Fluid Prescription.International journal of general medicine · 2023Review
- The Endothelial Glycocalyx and Neonatal Sepsis.International journal of molecular sciences · 2022Review
- A Dietary Supplement Containing Fucoidan Preserves Endothelial Glycocalyx through ERK/MAPK Signaling and Protects against Damage Induced by CKD Serum.International journal of molecular sciences · 2022Article
- Differential effects of vascular endothelial growth factor on glycocalyx of endothelial and tumor cells and potential targets for tumor metastasis.APL bioengineering · 2022Article
- Protection and rebuilding of the endothelial glycocalyx in sepsis - Science or fiction?Matrix biology plus · 2021Article
- The neurovascular extracellular matrix in health and disease.Experimental biology and medicine (Maywood, N.J.) · 2021Review
- The Glycocalyx and Its Role in Vascular Physiology and Vascular Related Diseases.Cardiovascular engineering and technology · 2021Review
- Vascular Endothelial Glycocalyx Plays a Role in the Obesity Paradox According to Intravital Observation.Frontiers in cardiovascular medicine · 2021Article
- Transcellular Model for Neutral and Charged Nanoparticles Across an In Vitro Blood-Brain Barrier.Cardiovascular engineering and technology · 2020Article
- Essential role and therapeutic targeting of the glomerular endothelial glycocalyx in lupus nephritis.JCI insight · 2020Article
- Endothelial Glycocalyx Layer Properties and Its Ability to Limit Leukocyte Adhesion.Biophysical journal · 2020Article
- Glycocalyx in Endotoxemia and Sepsis.The American journal of pathology · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The glycocalyx on the surface of endothelium lining blood vessel walls modulates vascular barrier function, cell adhesion and also serves as a mechano-sensor for blood flow. Reduction of glycocalyx has been reported in many diseases including atherosclerosis, inflammation, myocardial edema, and diabetes. The surface glycocalyx layer (SGL) is composed of proteoglycans and glycosaminoglycans, of which heparan sulfate is one of the most abundant. To quantify the SGL thickness on the microvessels of rat mesentery and mouse cremaster muscle in situ, we applied a single vessel cannulation and perfusion technique to directly inject FITC-anti-heparan sulfate into a group of microvessels for immuno-labeling the SGL. We also used anti-heparan sulfate for immuno-labeling the SGL on rat and mouse aortas ex vivo. High resolution confocal microscopy revealed that the thickness of the SGL on rat mesenteric capillaries and post-capillary venules is 0.9±0.1 μm and 1.2±0.3 μm, respectively; while the thickness of the SGL on mouse cremaster muscle capillaries and post-capillary venules is 1.5±0.1 μm and 1.5±0.2 μm, respectively. Surprisingly, there was no detectable SGL in either rat mesenteric or mouse cremaster muscle arterioles. The SGL thickness is 2.5±0.1 μm and 2.1±0.2 μm respectively, on rat and mouse aorta. In addition, we observed that the SGL is continuously and evenly distributed on the aorta wall but not on the microvessel wall.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.