ArticleGenome biology2012
Genome-wide analysis of the maternal-to-zygotic transition in Drosophila primordial germ cells.
Article in Genome biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 65 citations in OpenAlex.
- Article
- Leveraging CRISPR-Cas13d in an inducible knockdown system to interrogatebioRxiv : the preprint server for biology · 2025Article
- Review
- The maternal-to-zygotic transition: reprogramming of the cytoplasm and nucleus.Nature reviews. Genetics · 2025Review
- Smaug regulates germ plasm assembly and primordial germ cell number in Drosophila embryos.Science advances · 2024Article
- Review
- Structural basis for binding ofProceedings of the National Academy of Sciences of the United States of America · 2023Article
- RNA Helicase Vasa as a Multifunctional Conservative Regulator of Gametogenesis in Eukaryotes.Current issues in molecular biology · 2023Review
- Plasticity of Drosophila germ granules during germ cell development.PLoS biology · 2023Article
- Review
- Germline immortality relies on TRIM32-mediated turnover of a maternal mRNA activator inScience advances · 2022Article
- Maternal Piwi regulates primordial germ cell development to ensure the fertility of female progeny in Drosophila.Genetics · 2021Article
- Intrinsically disordered protein PID-2 modulates Z granules and is required for heritable piRNA-induced silencing in the Caenorhabditis elegans embryo.The EMBO journal · 2021Article
- Chemical Genetics: Manipulating the Germline with Small Molecules.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Molecular profiling of stem cell-like female germ line cells inBiology open · 2019Article
- Germ granules in Drosophila.Traffic (Copenhagen, Denmark) · 2019Review
- Noncanonical translation via deadenylated 3' UTRs maintains primordial germ cells.Nature chemical biology · 2018Article
- Tdrd6a Regulates the Aggregation of Buc into Functional Subcellular Compartments that Drive Germ Cell Specification.Developmental cell · 2018Article
- Evidence for a centrosome-attracting body like structure in germ-soma segregation during early development, in the urochordate Oikopleura dioica.BMC developmental biology · 2018Article
- The Smaug RNA-Binding Protein Is Essential for microRNA Synthesis During theG3 (Bethesda, Md.) · 2016Article
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
Abstract
backgroundDuring the maternal-to-zygotic transition (MZT) vast changes in the embryonic transcriptome are produced by a combination of two processes: elimination of maternally provided mRNAs and synthesis of new transcripts from the zygotic genome. Previous genome-wide analyses of the MZT have been restricted to whole embryos. Here we report the first such analysis for primordial germ cells (PGCs), the progenitors of the germ-line stem cells.
resultsWe purified PGCs from Drosophila embryos, defined their proteome and transcriptome, and assessed the content, scale and dynamics of their MZT. Transcripts encoding proteins that implement particular types of biological functions group into nine distinct expression profiles, reflecting coordinate control at the transcriptional and posttranscriptional levels. mRNAs encoding germ-plasm components and cell-cell signaling molecules are rapidly degraded while new transcription produces mRNAs encoding the core transcriptional and protein synthetic machineries. The RNA-binding protein Smaug is essential for the PGC MZT, clearing transcripts encoding proteins that regulate stem cell behavior, transcriptional and posttranscriptional processes. Computational analyses suggest that Smaug and AU-rich element binding proteins function independently to control transcript elimination.
conclusionsThe scale of the MZT is similar in the soma and PGCs. However, the timing and content of their MZTs differ, reflecting the distinct developmental imperatives of these cell types. The PGC MZT is delayed relative to that in the soma, likely because relief of PGC-specific transcriptional silencing is required for zygotic genome activation as well as for efficient maternal transcript clearance.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.