ArticlePloS one2012
Immuno-PCR--a new tool for paleomicrobiology: the plague paradigm.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 10 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
10 citing papers in PubMed, 32 citations in OpenAlex.
- Article
- Applications of polymerase chain reaction-based methods for the diagnosis of plague (Review).Experimental and therapeutic medicine · 2022Review
- Expression of Concern: Immuno-PCR-A New Tool for Paleomicrobiology: The Plague Paradigm.PloS one · 2022Article
- Yersinia pestis: the Natural History of Plague.Clinical microbiology reviews · 2020Review
- Ancient dental pulp: Masterpiece tissue for paleomicrobiology.Molecular genetics & genomic medicine · 2020Review
- Two thousand years of epidemics in Marseille and the Mediterranean Basin.New microbes and new infections · 2018Article
- Bacteriophage Sf6 Tailspike Protein for Detection ofViruses · 2018Article
- Paleoproteomics of the Dental Pulp: The plague paradigm.PloS one · 2017Article
- Environmental Microbial Forensics and Archaeology of Past Pandemics.Microbiology spectrum · 2017Review
- Surface plasmon resonance imaging of pathogens: the Yersinia pestis paradigm.BMC research notes · 2015Article
Corrections and comments
- Expression of concern · 2022-12-13Investigation by Journal/Publisher ·
- Commented on by
- Expression of concern
Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe cause of past plague pandemics was controversial but several research teams used PCR techniques and dental pulp as the primary material to reveal that they were caused by Yersinia pestis. However, the degradation of DNA limits the ability to detect ancient infections.
methodsWe used for the first time immuno-PCR to detect Yersinia pestis antigens; it can detect protein concentrations 70 times lower than the standard ELISA. After determining the cut-off value, we tested 34 teeth that were obtained from mass graves of plague, and compared previous PCR results with ELISA and immuno-PCR results.
resultsThe immuno-PCR technique was the most sensitive (14 out of 34) followed by the PCR technique (10 out of 34) and ELISA (3 out of 34). The combination of these three methods identified 18 out of 34 (53%) teeth as presumably being from people with the plague.
conclusionImmuno-PCR is specific (no false-positive samples were found) and more sensitive than the currently used method to detect antigens of ancient infections in dental pulp. The combination of three methods, ELISA, PCR and immuno-PCR, increased the capacity to identify ancient pathogens in dental pulp.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.