ArticlePloS one2012
Plasma cell-free DNA levels are elevated in acute Puumala hantavirus infection.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 38 citations in OpenAlex.
- β2 integrin mediates hantavirus-induced release of neutrophil extracellular traps.The Journal of experimental medicine · 2014Trial
- Harnessing viral footprints in circulating free DNA (cfDNA) for early cancer detection: A focus on liquid-biopsy-based screening.International journal of cancer · 2026Review
- Acute Pancreatitis and Leukemoid Reaction as the Presenting Manifestation of Hemorrhagic Fever with Renal Syndrome: A Case Report.Infection and drug resistance · 2024Article
- The Diagnostic, Prognostic, and Therapeutic Potential of Cell-Free DNA with a Special Focus on COVID-19 and Other Viral Infections.International journal of molecular sciences · 2023Review
- Circulating cell-free DNA in health and disease - the relationship to health behaviours, ageing phenotypes and metabolomics.GeroScience · 2023Article
- New Perspectives on the Importance of Cell-Free DNA Biology.Diagnostics (Basel, Switzerland) · 2022Review
- Severity Biomarkers in Puumala Hantavirus Infection.Viruses · 2021Review
- Article
- Coagulopathy in Acute Puumala Hantavirus Infection.Viruses · 2021Review
- Neutrophil Activation in Acute Hemorrhagic Fever With Renal Syndrome Is Mediated by Hantavirus-Infected Microvascular Endothelial Cells.Frontiers in immunology · 2018Article
- Neutrophil Extracellular Traps Go Viral.Frontiers in immunology · 2016Review
- Emerging blood-based biomarkers for detection of gastric cancer.World journal of gastroenterology · 2015Review
- Urinary Neutrophil Gelatinase-Associated Lipocalin (NGAL) and proteinuria predict severity of acute kidney injury in Puumala virus infection.BMC infectious diseases · 2015Article
- Hantavirus-induced disruption of the endothelial barrier: neutrophils are on the payroll.Frontiers in microbiology · 2015Article
- NK cell activation in human hantavirus infection explained by virus-induced IL-15/IL15Rα expression.PLoS pathogens · 2014Article
- Increased plasma cell-free DNA level during HTNV infection: correlation with disease severity and virus load.Viruses · 2014Article
- The impact of chronic kidney disease and short-term treatment with rosiglitazone on plasma cell-free DNA levels.PPAR research · 2014Article
- Uncovering the mysteries of hantavirus infections.Nature reviews. Microbiology · 2013Review
- Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionPuumala hantavirus (PUUV) causes a hemorrhagic fever with renal syndrome called nephropathia epidemica (NE). The aim of the present study was to evaluate plasma cell-free DNA (cf-DNA) levels and urinary cf-DNA excretion in acute NE as well as their associations with the severity of the disease.
methodsTotal plasma cf-DNA was quantified directly in plasma of 61 patients and urine of 20 patients with acute NE. We also carried out a qualitative high-sensitivity lab-on-a-chip DNA assay in 20 patients to elucidate the appearance of cf-DNA in plasma and urine.
resultsThe maximum plasma cf-DNA values taken during acute NE were significantly higher than the control values taken after the hospitalization period (median 1.33 µg/ml, range 0.94-3.29 µg/ml vs. median 0.77 µg/ml, range 0.55-0.99 µg/ml, P<0.001). The maximum plasma cf-DNA levels correlated positively with maximum blood leukocyte count (r = 0.388, P = 0.002) and the length of hospital stay (r = 0.376, P = 0.003), and inversely with minimum blood platelet count (r = -0.297, P = 0.020). Qualitative analysis of plasma cf-DNA revealed that in most of the patients cf-DNA displayed a low-molecular weight appearance, corresponding to the size of apoptotic DNA (150-200 bp). The visually graded maximum cf-DNA band intensity correlated positively with the maximum quantity of total plasma cf-DNA (r = 0.513, P = 0.021). Maximum urinary excretion of cf-DNA in turn was not markedly increased during the acute phase of NE and did not correlate with any of the variables reflecting severity of the disease or with the maximum plasma cf-DNA level.
conclusionsThe plasma levels of cf-DNA are elevated during acute PUUV infection and correlate with the apoptotic cf-DNA-band intensity. The plasma cf-DNA concentration correlates with some variables reflecting the severity of the disease. The urinary excretion of cf-DNA does not reflect the degree of inflammation in the kidney.
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