ArticlePloS one2012
CTCF mediates the cell-type specific spatial organization of the Kcnq5 locus and the local gene regulation.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- Hidden origami inmBio · 2025Article
- STAT3 and SOX-5 induce BRG1-mediated chromatin remodeling of RORCE2 in Th17 cells.Communications biology · 2024Article
- Acute depletion of CTCF rewires genome-wide chromatin accessibility.Genome biology · 2021Article
- SOX-5 activates a novel RORγt enhancer to facilitate experimental autoimmune encephalomyelitis by promoting Th17 cell differentiation.Nature communications · 2021Article
- Mutation hotspots at CTCF binding sites coupled to chromosomal instability in gastrointestinal cancers.Nature communications · 2018Article
- 17q21 asthma-risk variants switch CTCF binding and regulate IL-2 production by T cells.Nature communications · 2016Article
- DNA methylation reactivates GAD1 expression in cancer by preventing CTCF-mediated polycomb repressive complex 2 recruitment.Oncogene · 2016Article
- Epigenome-wide effects of vitamin D and their impact on the transcriptome of human monocytes involve CTCF.Nucleic acids research · 2016Article
- Preferential association of a functional variant in complement receptor 2 with antibodies to double-stranded DNA.Annals of the rheumatic diseases · 2016Article
- An enhancer-blocking element regulates the cell-specific expression of alcohol dehydrogenase 7.Gene · 2014Article
- Comparative analysis of 4C-Seq data generated from enzyme-based and sonication-based methods.BMC genomics · 2013Article
- Chromatin insulators: linking genome organization to cellular function.Molecular cell · 2013Review
- Identification and analysis of pig chimeric mRNAs using RNA sequencing data.BMC genomics · 2012Article
- Chromatin loops, gene positioning, and gene expression.Frontiers in genetics · 2012Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chromatin loops play important roles in the dynamic spatial organization of genes in the nucleus. Growing evidence has revealed that the multivalent functional zinc finger protein CCCTC-binding factor (CTCF) is a master regulator of genome spatial organization, and mediates the ubiquitous chromatin loops within the genome. Using circular chromosome conformation capture (4C) methodology, we discovered that CTCF may be a master organizer in mediating the spatial organization of the kcnq5 gene locus. We characterized the cell-type specific spatial organization of the kcnq5 gene locus mediated by CTCF in detail using chromosome conformation capture (3C) and 3C-derived techniques. Cohesion also participated in mediating the organization of this locus. RNAi-mediated knockdown of CTCF sharply diminished the interaction frequencies between the chromatin loops of the kcnq5 gene locus and down-regulated local gene expression. Functional analysis showed that the interacting chromatin loops of the kcnq5 gene locus can repress the gene expression in a luciferase reporter assay. These interacting chromatin fragments were a series of repressing elements whose contacts were mediated by CTCF. Therefore, these findings suggested that the dynamical spatial organization of the kcnq5 locus regulates local gene expression.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.