ArticlePloS one2012
Analysis of miRNA and mRNA expression profiles highlights alterations in ionizing radiation response of human lymphocytes under modeled microgravity.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
60 citing papers in PubMed, 1 synthesis or guideline pooled it, 101 citations in OpenAlex.
- Candidate gene biodosimetry markers of exposure to external ionizing radiation in human blood: A systematic review.PloS one · 2018Pooled it
- Rice and shine: a review of miRNA-mediated responses to abiotic stress inFrontiers in plant science · 2026Review
- Exploring molecular and modular insights into space ionizing radiation effects through heterogeneous gene regulatory networks.NPJ microgravity · 2025Article
- Ionizing radiation effects on blood-derived extracellular vesicles: insights into miR-34a-5p-mediated cellular responses and biomarker potential.Cell communication and signaling : CCS · 2024Article
- NASA GeneLab derived microarray studies of Mus musculus and Homo sapiens organisms in altered gravitational conditions.NPJ microgravity · 2024Review
- Morphologic and genomic changes of thyroid cancer cell lines exposed to conditions of simulated microgravity.NPJ microgravity · 2024Article
- Translating genetic findings to epigenetics: identifying the mechanisms associated with aging after high-radiation exposure on earth and in space.Frontiers in public health · 2024Article
- Analysis of Radiation Toxicity in Mammalian Cells Stably Transduced with MitochondrialInternational journal of molecular sciences · 2023Article
- Establishing a genomic radiation-age association for space exploration supplements lung disease differentiation.Frontiers in public health · 2023Article
- A Current Overview of the Biological Effects of Combined Space Environmental Factors in Mammals.Frontiers in cell and developmental biology · 2022Review
- MicroRNAs as Biomarkers for Ionizing Radiation Injury.Frontiers in cell and developmental biology · 2022Review
- A 5-Pathway Signature Predicts Prognosis Based on Immune-Derived lncRNAs in Patients with Breast Cancer.Journal of oncology · 2022Article
- Cancer Studies under Space Conditions: Finding Answers Abroad.Biomedicines · 2021Review
- Genomic Changes Driven by Radiation-Induced DNA Damage and Microgravity in Human Cells.International journal of molecular sciences · 2021Review
- A Perspective of Epigenetic Regulation in Radiotherapy.Frontiers in cell and developmental biology · 2021Review
- The individual and combined effects of spaceflight radiation and microgravity on biologic systems and functional outcomes.Journal of environmental science and health. Part C, Toxicology and carcinogenesis · 2021Article
- Combined Environment Simulator for Low-Dose-Rate Radiation and Partial Gravity of Moon and Mars.Life (Basel, Switzerland) · 2020Article
- Additive effects of simulated microgravity and ionizing radiation in cell death, induction of ROS and expression of RAC2 in human bronchial epithelial cells.NPJ microgravity · 2020Article
- MicroRNAs Responding to Space Radiation.International journal of molecular sciences · 2020Review
- Changes in miRNA expressions in the injured small intestine of mice following high‑dose radiation exposure.Molecular medicine reports · 2020Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIonizing radiation (IR) can be extremely harmful for human cells since an improper DNA-damage response (DDR) to IR can contribute to carcinogenesis initiation. Perturbations in DDR pathway can originate from alteration in the functionality of the microRNA-mediated gene regulation, being microRNAs (miRNAs) small noncoding RNA that act as post-transcriptional regulators of gene expression. In this study we gained insight into the role of miRNAs in the regulation of DDR to IR under microgravity, a condition of weightlessness experienced by astronauts during space missions, which could have a synergistic action on cells, increasing the risk of radiation exposure. METHODOLOGY/PRINCIPAL
findingsWe analyzed miRNA expression profile of human peripheral blood lymphocytes (PBL) incubated for 4 and 24 h in normal gravity (1 g) and in modeled microgravity (MMG) during the repair time after irradiation with 0.2 and 2Gy of γ-rays. Our results show that MMG alters miRNA expression signature of irradiated PBL by decreasing the number of radio-responsive miRNAs. Moreover, let-7i*, miR-7, miR-7-1*, miR-27a, miR-144, miR-200a, miR-598, miR-650 are deregulated by the combined action of radiation and MMG. Integrated analyses of miRNA and mRNA expression profiles, carried out on PBL of the same donors, identified significant miRNA-mRNA anti-correlations of DDR pathway. Gene Ontology analysis reports that the biological category of "Response to DNA damage" is enriched when PBL are incubated in 1 g but not in MMG. Moreover, some anti-correlated genes of p53-pathway show a different expression level between 1 g and MMG. Functional validation assays using luciferase reporter constructs confirmed miRNA-mRNA interactions derived from target prediction analyses. CONCLUSIONS/SIGNIFICANCE: On the whole, by integrating the transcriptome and microRNome, we provide evidence that modeled microgravity can affects the DNA-damage response to IR in human PBL.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.