ArticlePloS one2012
Highly parallel genome-wide expression analysis of single mammalian cells.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- LINCATRA: Two-cycle method to amplify RNA for transcriptome analysis from formalin-fixed paraffin-embedded tissue.Heliyon · 2024Article
- Article
- Improving single-cell transcriptome sequencing efficiency with a microfluidic phase-switch device.The Analyst · 2019Article
- Single-cell transcriptomes reveal the mechanism for a breast cancer prognostic gene panel.Oncotarget · 2018Article
- Relapse pathway of glioblastoma revealed by single-cell molecular analysis.Carcinogenesis · 2018Article
- Single-Cell Digital Lysates Generated by Phase-Switch Microfluidic Device Reveal Transcriptome Perturbation of Cell Cycle.ACS nano · 2018Article
- Review
- Article
- Epigenomic Reprogramming of Adult Cardiomyocyte-Derived Cardiac Progenitor Cells.Scientific reports · 2015Article
- The Future of Prenatal Diagnosis and Screening.Journal of clinical medicine · 2014Article
- Low-coverage single-cell mRNA sequencing reveals cellular heterogeneity and activated signaling pathways in developing cerebral cortex.Nature biotechnology · 2014Article
- Investigating evolutionary perspective of carcinogenesis with single-cell transcriptome analysis.Chinese journal of cancer · 2013Article
- Comparison of RNA- or LNA-hybrid oligonucleotides in template-switching reactions for high-speed sequencing library preparation.BMC genomics · 2013Article
- The transcriptional architecture of early human hematopoiesis identifies multilevel control of lymphoid commitment.Nature immunology · 2013Article
- Quartz-Seq: a highly reproducible and sensitive single-cell RNA sequencing method, reveals non-genetic gene-expression heterogeneity.Genome biology · 2013Article
- Suppression of artifacts and barcode bias in high-throughput transcriptome analyses utilizing template switching.Nucleic acids research · 2013Article
- Population transcriptomics with single-cell resolution: a new field made possible by microfluidics: a technology for high throughput transcript counting and data-driven definition of cell types.BioEssays : news and reviews in molecular, cellular and developmental biology · 2013Article
- Is central dogma a global property of cellular information flow?Frontiers in physiology · 2012Article
- Molecular characterization of heterogeneous mesenchymal stem cells with single-cell transcriptomes.Biotechnology advancesReview
Corrections and comments
- Erratum issuedLoring, Jeanne F [added]
Authors and funding
16 authors.
Funding
Abstract
backgroundWe have developed a high-throughput amplification method for generating robust gene expression profiles using single cell or low RNA inputs. METHODOLOGY/PRINCIPAL
findingsThe method uses tagged priming and template-switching, resulting in the incorporation of universal PCR priming sites at both ends of the synthesized cDNA for global PCR amplification. Coupled with a whole-genome gene expression microarray platform, we routinely obtain expression correlation values of R(2)~0.76-0.80 between individual cells and R(2)~0.69 between 50 pg total RNA replicates. Expression profiles generated from single cells or 50 pg total RNA correlate well with that generated with higher input (1 ng total RNA) (R(2)~0.80). Also, the assay is sufficiently sensitive to detect, in a single cell, approximately 63% of the number of genes detected with 1 ng input, with approximately 97% of the genes detected in the single-cell input also detected in the higher input. CONCLUSIONS/SIGNIFICANCE: In summary, our method facilitates whole-genome gene expression profiling in contexts where starting material is extremely limiting, particularly in areas such as the study of progenitor cells in early development and tumor stem cell biology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.