ArticleIranian journal of parasitology2011
The Effect of Garlic Extract on Expression of INFγ And Inos Genes in Macrophages Infected with Leishmania major.
Article in Iranian journal of parasitology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
10 citing papers in PubMed, 19 citations in OpenAlex.
- The Safety and Efficacy of an Immunomodulator Garlic-Based Cream (LeishG1) for Treating Uncomplicated Cutaneous Leishmaniasis: A Single-Blind, Multi-Center Study.Health science reports · 2025Article
- Herbal and Spice Additives in Functional Confectionery Products: A Review.Molecules (Basel, Switzerland) · 2025Review
- Wound healing activity of green synthesized copper nanoparticles through cell proliferation-migration, antimicrobial effects, and nitric oxide triggering.Archives of Razi Institute · 2024Article
- Leishmanicidal and immunomodulatory activities of the formononetin (a natural isoflavone) against Leishmania tropica.BMC research notes · 2023Article
- Medicinal plants with promising antileishmanial activity in Iran: a systematic review and meta-analysis.Annals of medicine and surgery (2012) · 2017Review
- Antileishmanial and Immunomodulatory Activity of Allium sativum (Garlic): A Review.Journal of evidence-based complementary & alternative medicine · 2017Review
- Immunomodulation and anti-inflammatory effects of garlic compounds.Journal of immunology research · 2015Review
- The antitrichomonal efficacy of garlic and metronidazole against Trichomonas gallinae infecting domestic pigeons.Parasitology research · 2014Article
- Therapeutic Effect of Hedera helix Alcoholic Extract Against Cutaneous Leishmaniasis Caused by Leishmania major in Balb/c Mice.Jundishapur journal of microbiology · 2014Article
- Exploring the role of medicinal plant-based immunomodulators for effective therapy of leishmaniasis.Frontiers in immunology · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe study was aimed to show the effect of molecular mechanism of Aqueous Garlic Extract (AGE) on expression of IFNγ and iNOS genes in Leishmania major.
methodsLeishmania major promastigotes (MRHO/IR/75/ER) were added to the in-vitro cultured J774 cell line, the cells were incubated for 72 hours. Various concentrations of garlic extract (9.25, 18.5, 37, 74, 148 mg/ml) were added to the infected cells. MTT assay was applied for cellular proliferation. After 72 hours of incubation, supernatants were collected and total RNA was extracted from the infected cells. The express of IFNγ and iNOS genes were studied by RT-PCR method.
resultsThe colorimetric MTT assay after 3 days of incubation showed cytotoxic effect of garlic extract with an IC50 of 37 mg/ml. In addition, IFNγ and iNOS genes expression by RT-PCR indicated that garlic extract lead to over expression of these genes in J774 cell line infected with L. major.
conclusionGarlic extract exerts cytotoxic effect on infected J774 cell line. In addition, the hypothesis that garlic can improve cellular immunity with raising the expression of IFNγ and of iNOS genes confirmed.
Indexed as
Identifiers
22347300PMC3279889W1931213484What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.