ArticleIranian journal of parasitology2011
Development of sensitive detection of cryptosporidium and giardia from surface water in iran.
Article in Iranian journal of parasitology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed, 13 citations in OpenAlex.
- Detection ofMicroorganisms · 2022Review
- Intestinal protozoan and helminthic infections among hemodialysis and cancer patients.Parasitology research · 2020Article
- Report of Giardia assemblages and giardiasis in residents of Guilan province-Iran.Parasitology research · 2020Article
- Genotyping of Giardia lamblia and Entamoeba spp from river waters in Iran.Parasitology research · 2015Article
- Detection of parasitic particles in domestic and urban wastewaters and assessment of removal efficiency of treatment plants in Tehran, Iran.Journal of environmental health science & engineering · 2015Article
- Microscopic and Molecular Detection ofIranian journal of parasitologyArticle
- Detection of Acanthamoeba and Toxoplasma in River Water Samples by Molecular Methods in Iran.Iranian journal of parasitologyArticle
- Isolation of Small Number of Cryptosporidium parvum Oocyst Using Immunochromatography.Iranian journal of parasitologyArticle
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe protozoan parasites Cryptosporidium spp. and Giardia are known to occur widely in both raw and drinking waters. They are two of the causative agents of waterborne outbreaks of gastroenteritis throughout the world. In the present study, a PCR assay and FA were developed for detection of Cryptosporidium oocysts and Giardia cyst in environmental samples.
methodsWe have detected Cryptosporidium spp. oocysts and Giardia cysts in seeded and unseeded environmental water samples by PCR method. Water samples were spiked with oocysts (50, 100,300,500) and filtrated with a 1.2-µm pore size cellulose nitrate and follow by DNA extraction and purification by QIAamp DNA mini kit. Nested-PCR assay amplified an 850 bp fragment of 18s rRNA gene specific for Cryptosporidium and 435 bp fragment of glutamate dehydrogenase (GDH) target gene for Giardia. Also many river water from north of Iran, be checked by these methods.
resultsCryptosporidium and Giardia DNAs were detected in seeded water sample and Giardia was detected in all 5 water samples from river in north of Iran by nested- PCR and FA. Also in one river water sample, Cryptosporidium was detected.
conclusionThis protocol is effective for detection of these waterborne parasites in treated and untreated water samples. This study can also serve as a platform for further investigations and research water source in Iran.
Indexed as
Identifiers
22347296PMC3279887W2146562632What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.