ArticleJournal of immunology (Baltimore, Md. : 1950)2012
Germline deletion of Igh 3' regulatory region elements hs 5, 6, 7 (hs5-7) affects B cell-specific regulation, rearrangement, and insulation of the Igh locus.
Article in Journal of immunology (Baltimore, Md. : 1950), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 47 citations in OpenAlex.
- Maturation of HIV-1 neutralizing antibodies in a germinal center conditional expression mouse model.PLoS pathogens · 2026Article
- Igh nuclear speckle association promotes V(D)J recombination.Nature communications · 2026Article
- Core regions in immunoglobulin heavy chain enhancers essential for survival of non-Hodgkin lymphoma cells are identified by a CRISPR interference screen.Haematologica · 2024Article
- Core enhancers of the 3'RR optimize IgH nuclear position and loop conformation for successful oriented class switch recombination.Nucleic acids research · 2024Article
- The immunoglobulin heavy chain super enhancer controls class switch recombination in developing B cells.Scientific reports · 2024Article
- Lineage- and stage-specific activity of antigen receptor gene enhancers during lymphocyte development.Frontiers in epigenetics and epigenomics · 2024Review
- Intra- and interchromosomal contact mapping reveals the Igh locus has extensive conformational heterogeneity and interacts with B-lineage genes.Cell reports · 2023Article
- Evolutive emergence and divergence of an Ig regulatory node: An environmental sensor getting cues from the aryl hydrocarbon receptor?Frontiers in immunology · 2023Review
- Efficient human-like antibody repertoire and hybridoma production in trans-chromosomic mice carrying megabase-sized human immunoglobulin loci.Nature communications · 2022Article
- Contribution of Immunoglobulin Enhancers to B Cell Nuclear Organization.Frontiers in immunology · 2022Review
- An insulator that regulates chromatin extrusion and class switch recombination.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Noncoding RNA processing by DIS3 regulates chromosomal architecture and somatic hypermutation in B cells.Nature genetics · 2021Article
- Physiological role of the 3'IgH CBEs super-anchor in antibody class switching.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Long-Range Control of Class Switch Recombination by Transcriptional Regulatory Elements.Frontiers in immunology · 2021Review
- Genome Topology Control of Antigen Receptor Gene Assembly.Journal of immunology (Baltimore, Md. : 1950) · 2020Review
- Noncoding RNA transcription alters chromosomal topology to promote isotype-specific class switch recombination.Science immunology · 2020Article
- Mouse Models ofFrontiers in immunology · 2020Review
- Two Successive Inversional Vβ Rearrangements on a SingleJournal of immunology (Baltimore, Md. : 1950) · 2020Article
- The murine IgH locus contains a distinct DNA sequence motif for the chromatin regulatory factor CTCF.The Journal of biological chemistry · 2019Article
- Complex sex-biased antibody responses: estrogen receptors bind estrogen response elements centered within immunoglobulin heavy chain gene enhancers.International immunology · 2019Article
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Authors and funding
14 authors at 2 institutions in 1 country.
Funding
Abstract
Regulatory elements located within an ∼28-kb region 3' of the Igh gene cluster (3' regulatory region) are required for class switch recombination and for high levels of IgH expression in plasma cells. We previously defined novel DNase I hypersensitive sites (hs) 5, 6, 7 immediately downstream of this region. The hs 5-7 region (hs5-7) contains a high density of binding sites for CCCTC-binding factor (CTCF), a zinc finger protein associated with mammalian insulator activity, and is an anchor for interactions with CTCF sites flanking the D(H) region. To test the function of hs5-7, we generated mice with an 8-kb deletion encompassing all three hs elements. B cells from hs5-7 knockout (KO) (hs5-7KO) mice showed a modest increase in expression of the nearest downstream gene. In addition, Igh alleles in hs5-7KO mice were in a less contracted configuration compared with wild-type Igh alleles and showed a 2-fold increase in the usage of proximal V(H)7183 gene families. Hs5-7KO mice were essentially indistinguishable from wild-type mice in B cell development, allelic regulation, class switch recombination, and chromosomal looping. We conclude that hs5-7, a high-density CTCF-binding region at the 3' end of the Igh locus, impacts usage of V(H) regions as far as 500 kb away.
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