ArticleGenes & development2012
Sumoylation of transcription factor Gcn4 facilitates its Srb10-mediated clearance from promoters in yeast.
Article in Genes & development, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 53 citations in OpenAlex.
- Article
- Cyclin-dependent kinases: Masters of the eukaryotic universe.Wiley interdisciplinary reviews. RNA · 2023Review
- Genome-Wide Identification and Functional Analysis of the bZIP Transcription Factor Family in Rice Bakanae Disease Pathogen,International journal of molecular sciences · 2022Article
- The Candida albicans Cdk8-dependent phosphoproteome reveals repression of hyphal growth through a Flo8-dependent pathway.PLoS genetics · 2022Article
- Mechanism and function of DNA replication-independent DNA-protein crosslink repair via the SUMO-RNF4 pathway.The EMBO journal · 2021Article
- Dynamic sumoylation of promoter-bound general transcription factors facilitates transcription by RNA polymerase II.PLoS genetics · 2021Article
- SUMO and Transcriptional Regulation: The Lessons of Large-Scale Proteomic, Modifomic and Genomic Studies.Molecules (Basel, Switzerland) · 2021Review
- SUMO is a pervasive regulator of meiosis.eLife · 2021Article
- CiBER-seq dissects genetic networks by quantitative CRISPRi profiling of expression phenotypes.Science (New York, N.Y.) · 2020Article
- Regulation of Skn7-dependent, oxidative stress-induced genes by the RNA polymerase II-CTD phosphatase, Fcp1, and Mediator kinase subunit, Cdk8, in yeast.The Journal of biological chemistry · 2019Article
- Sumoylation of DNA-bound transcription factor Sko1 prevents its association with nontarget promoters.PLoS genetics · 2019Article
- SUMO Chains Rule on Chromatin Occupancy.Frontiers in cell and developmental biology · 2019Review
- MPK1/SLT2 Links Multiple Stress Responses with Gene Expression in Budding Yeast by Phosphorylating Tyr1 of the RNAP II CTD.Molecular cell · 2017Article
- Regulation of transcription factors by sumoylation.Transcription · 2017Review
- Nup100 regulatesRNA (New York, N.Y.) · 2017Article
- TORC1-dependent sumoylation of Rpc82 promotes RNA polymerase III assembly and activity.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Chromatin Association of Gcn4 Is Limited by Post-translational Modifications Triggered by its DNA-Binding in Saccharomyces cerevisiae.Genetics · 2016Article
- Mediator Undergoes a Compositional Change during Transcriptional Activation.Molecular cell · 2016Article
- Interaction of Gcn4 with target gene chromatin is modulated by proteasome function.Molecular biology of the cell · 2016Article
- Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The small ubiquitin-related modifier (SUMO) is a conserved factor that post-translationally regulates proteins involved in many cellular processes, including gene transcription. We previously demonstrated that promoter-bound factors become sumoylated during activation of inducible genes in yeast, but the identity of these factors, and the role of sumoylation in their function, was unknown. Here we show that the transcriptional activator Gcn4 is sumoylated on two specific lysine residues and in a manner that depends on its ability to bind DNA, indicating that sumoylation occurs after Gcn4 binding to target promoters. Importantly, this functions to facilitate the subsequent removal of the activator from these promoters after recruitment of RNA polymerase II, which can prevent inappropriate transcription of target genes. Furthermore, we show that clearance of sumoylated Gcn4 requires the protein kinase and Mediator complex subunit Srb10, linking activator removal with target gene transcription. Our study demonstrates an unexpected role for protein sumoylation in the process of transcriptional activation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.