ArticleNature protocols2012
Construction of BIBAC and BAC libraries from a variety of organisms for advanced genomics research.
Article in Nature protocols, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT06924697 (Effect of Glucagon-like Peptide-1), which is not on this map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Effect of Glucagon-like Peptide-1 (GLP-1) Receptor Agonist Stimulation on Smoking Consumption in Type 2 Diabetes Patients: Study Protocol of a Randomized, Parallel -Controlled Clinical Trial
Who cites it
13 citing papers in PubMed.
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- An assessment of transgenomics as a tool for gene discovery in Populus euphratica Oliv.Plant molecular biology · 2018Article
- Oak genome reveals facets of long lifespan.Nature plants · 2018Article
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- MISSA 2.0: an updated synthetic biology toolbox for assembly of orthogonal CRISPR/Cas systems.Scientific reports · 2017Article
- Targeted isolation and cloning of 100-kb microbial genomic sequences by Cas9-assisted targeting of chromosome segments.Nature protocols · 2016Article
- Cas9-Assisted Targeting of CHromosome segments CATCH enables one-step targeted cloning of large gene clusters.Nature communications · 2015Article
- Fungal artificial chromosomes for mining of the fungal secondary metabolome.BMC genomics · 2015Article
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- Construction of a plant-transformation-competent BIBAC library and genome sequence analysis of polyploid Upland cotton (Gossypium hirsutum L.).BMC genomics · 2013Article
- Preparation of megabase-sized DNA from a variety of organisms using the nuclei method for advanced genomics research.Nature protocols · 2012Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Large-insert BAC (bacterial artificial chromosome) and BIBAC (binary BAC) libraries are essential for modern genomics research for all organisms. We helped pioneer the BAC and BIBAC technologies, and by using them we have constructed hundreds of BAC and BIBAC libraries for different species of plants, animals, marine animals, insects, algae and microbes. These libraries have been used globally for different aspects of genomics research. Here we describe the procedure with the latest improvements that we have made and used for construction of BIBAC libraries. The procedure includes the preparation of BIBAC vectors, the preparation of clonable fragments of the desired size from the source DNA, the construction and transformation of BIBACs and, finally, the characterization and assembly of BIBAC libraries. We also specify the modifications necessary for construction of BAC libraries using the protocol. The entire protocol takes ∼7 d.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.