ArticleAnalytical biochemistry2012
A benchmarked protein microarray-based platform for the identification of novel low-affinity extracellular protein interactions.
Article in Analytical biochemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 51 citations in OpenAlex.
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- An Automated End-to-End Workflow for Production of Secreted Proteins in Transfected Mammalian Cells.bioRxiv : the preprint server for biology · 2025Article
- Eggs-posed: revision of Schistosoma mansoni venom allergen-like proteins unveils new genes and offers new insights into egg-host interactions.BMC genomics · 2025Article
- A panel of recombinantmBio · 2024Article
- No evidence for a direct extracellular interaction between human Fc receptor-like 3 (MAIA) and the sperm ligand IZUMO1.Science advances · 2024Article
- Complement receptor 1 is the human erythrocyte receptor forProceedings of the National Academy of Sciences of the United States of America · 2024Article
- The structure of a Plasmodium vivax Tryptophan Rich Antigen domain suggests a lipid binding function for a pan-Plasmodium multi-gene family.Nature communications · 2023Article
- LRRC15 mediates an accessory interaction with the SARS-CoV-2 spike protein.PLoS biology · 2023Article
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- AVEXIS technology identifies novel platelet-leukocyte binding partners including CD148-CD300a.Blood advances · 2021Article
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- No evidence for basigin/CD147 as a direct SARS-CoV-2 spike binding receptor.Scientific reports · 2021Article
- A Human IgSF Cell-Surface Interactome Reveals a Complex Network of Protein-Protein Interactions.Cell · 2020Article
- Rapid and sensitive large-scale screening of low affinity extracellular receptor protein interactions by using reaction induced inhibition of Gaussia luciferase.Scientific reports · 2020Article
- A panel of recombinant proteins from human-infective Plasmodium species for serological surveillance.Malaria journal · 2020Article
- High-Content Imaging for Large-Scale Detection of Low-Affinity Extracellular Protein Interactions.SLAS discovery : advancing life sciences R & D · 2019Article
- A defined mechanistic correlate of protection against Plasmodium falciparum malaria in non-human primates.Nature communications · 2019Article
- KILchip v1.0: A NovelFrontiers in immunology · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Low-affinity extracellular protein interactions are critical for cellular recognition processes, but existing methods to detect them are limited in scale, making genome-wide interaction screens technically challenging. To address this, we report here the miniaturization of the AVEXIS (avidity-based extracellular interaction screen) assay by using protein microarray technology. To achieve this, we have developed protein tags and sample preparation methods that enable the parallel purification of hundreds of recombinant proteins expressed in mammalian cells. We benchmarked the protein microarray-based assay against a set of known quantified receptor-ligand pairs and show that it is sensitive enough to detect even very weak interactions that are typical of this class of interactions. The increase in scale enables interaction screening against a dilution series of immobilized proteins on the microarray enabling the observation of saturation binding behaviors to show interaction specificity and also the estimation of interaction affinities directly from the primary screen. These methodological improvements now permit screening for novel extracellular receptor-ligand interactions on a genome-wide scale.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.