ReviewProgress in brain research2012
Optogenetic reporters: Fluorescent protein-based genetically encoded indicators of signaling and metabolism in the brain.
Review in Progress in brain research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed.
- Genetically Encoded Metabolic Sensors to Study Retina Metabolism.Advances in experimental medicine and biology · 2025Review
- Excitation-Dependent pKa Extends the Sensing Range of Fluorescence Lifetime pH Sensors.Sensors (Basel, Switzerland) · 2024Article
- A genetically encoded fluorescent biosensor for detecting itaconate with subcellular resolution in living macrophages.Nature communications · 2022Article
- A Circuit-Based Information Approach to Substance Abuse Research.Trends in neurosciences · 2021Review
- Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular physiology.Nature communications · 2020Article
- Quantitative Imaging of Changes in Astrocytic and Neuronal Adenosine Triphosphate Using Two Different Variants of ATeam.Frontiers in cellular neuroscience · 2020Article
- Simultaneous voltage and calcium imaging and optogenetic stimulation with high sensitivity and a wide field of view.Biomedical optics express · 2019Article
- Neurovascular Network Explorer 2.0: A Simple Tool for Exploring and Sharing a Database of Optogenetically-evoked Vasomotion in Mouse Cortex In Vivo.Journal of visualized experiments : JoVE · 2018Article
- Guidelines on experimental methods to assess mitochondrial dysfunction in cellular models of neurodegenerative diseases.Cell death and differentiation · 2018Review
- Article
- Ultrawidefield microscope for high-speed fluorescence imaging and targeted optogenetic stimulation.Biomedical optics express · 2017Article
- Na/K pump inactivation, subsarcolemmal Na measurements, and cytoplasmic ion turnover kinetics contradict restricted Na spaces in murine cardiac myocytes.The Journal of general physiology · 2017Article
- Nondestructive nanostraw intracellular sampling for longitudinal cell monitoring.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Illuminating Brain Activities with Fluorescent Protein-Based Biosensors.Chemosensors (Basel, Switzerland) · 2017Article
- Genetically Encoded Voltage Indicators: Opportunities and Challenges.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Review
- Biosensor reveals multiple sources for mitochondrial NAD⁺.Science (New York, N.Y.) · 2016Article
- Review
- Optogenetic tools for modulating and probing the epileptic network.Epilepsy research · 2015Review
- NH4(+) triggers the release of astrocytic lactate via mitochondrial pyruvate shunting.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Culture and adenoviral infection of sinoatrial node myocytes from adult mice.American journal of physiology. Heart and circulatory physiology · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Fluorescent protein technology has evolved to include genetically encoded biosensors that can monitor levels of ions, metabolites, and enzyme activities as well as protein conformation and even membrane voltage. They are well suited to live-cell microscopy and quantitative analysis, and they can be used in multiple imaging modes, including one- or two-photon fluorescence intensity or lifetime microscopy. Although not nearly complete, there now exists a substantial set of genetically encoded reporters that can be used to monitor many aspects of neuronal and glial biology, and these biosensors can be used to visualize synaptic transmission and activity-dependent signaling in vitro and in vivo. In this review, we present an overview of design strategies for engineering biosensors, including sensor designs using circularly permuted fluorescent proteins and using fluorescence resonance energy transfer between fluorescent proteins. We also provide examples of indicators that sense small ions (e.g., pH, chloride, zinc), metabolites (e.g., glutamate, glucose, ATP, cAMP, lipid metabolites), signaling pathways (e.g., G protein-coupled receptors, Rho GTPases), enzyme activities (e.g., protein kinase A, caspases), and reactive species. We focus on examples where these genetically encoded indicators have been applied to brain-related studies and used with live-cell fluorescence microscopy.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.