ArticleMethods in enzymology2012
Multiphoton microscopy applied for real-time intravital imaging of bacterial infections in vivo.
Article in Methods in enzymology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 19 citations in OpenAlex.
- Physical Properties of E143 Food Dye as a New Organic Semiconductor Nanomaterial.Nanomaterials (Basel, Switzerland) · 2023Article
- Intravital Multiphoton Microscopy as a Tool for Studying Renal Physiology, Pathophysiology and Therapeutics.Frontiers in physiology · 2022Review
- Kidney intercalated cells are phagocytic and acidify internalized uropathogenic Escherichia coli.Nature communications · 2021Article
- Unraveling the host's immune response to infection: Seeing is believing.Journal of leukocyte biology · 2019Review
- High Resolution Intravital Imaging of the Renal Immune Response to Injury and Infection in Mice.Frontiers in immunology · 2019Article
- Intravital imaging of the kidney.Methods (San Diego, Calif.) · 2017Article
- Intravital multiphoton microscopy as a tool for studying renal physiology and pathophysiology.Methods (San Diego, Calif.) · 2017Review
- Intravital imaging of the kidney in a rat model of salt-sensitive hypertension.American journal of physiology. Renal physiology · 2017Article
- The multifaceted role of the renal microvasculature during acute kidney injury.Pediatric nephrology (Berlin, Germany) · 2016Review
- Renal Hemodynamics in AKI: In Search of New Treatment Targets.Journal of the American Society of Nephrology : JASN · 2016Article
- Therapeutic translation in acute kidney injury: the epithelial/endothelial axis.The Journal of clinical investigation · 2014Review
- Letter to the editor: "Quantifying albumin permeability with multiphoton microscopy: why the difference?".American journal of physiology. Renal physiology · 2014Article
- Finding the bottom and using it: Offsets and sensitivity in the detection of low intensity values in vivo with 2-photon microscopy.Intravital · 2014Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
To understand the underlying mechanisms of bacterial infections, researchers have for long addressed the molecular interactions occurring when the bacterium interacts with host target cells. In these studies, primarily based on in vitro systems, molecular details have been revealed along with increased knowledge regarding the general infection process. With the recent advancements in in vivo imaging techniques, we are now in a position to bridge a transition from classical minimalistic in vitro approaches to allow infections to be studied in its native complexity-the live organ. Techniques such as multiphoton microscopy (MPM) allow cellular-level visualization of the dynamic infection process in real time within the living host. Studies in which all interplaying factors, such as the influences of the immune, lymphatic, and vascular systems can be accounted for, are likely to provide new insights to our current understanding of the infection process. MPM imaging becomes extra powerful when combined with advanced surgical procedure, allowing studies of the illusive early hours of infection. In this chapter, our intention is to provide a general view on how to design and carry out intravital imaging of a bacterial infection. While exemplifying this using a spatiotemporally well-controlled uropathogenic Escherichia coli (UPEC) infection in rat kidneys, we hope to provide the reader with general considerations that can be adapted to other bacterial infections in organs other than the kidney.
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Registered trials
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