ArticleBMC research notes2012
The isolation and characterisation of the wheat molecular ZIPper I homologue, TaZYP1.
Article in BMC research notes, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 22 citations in OpenAlex.
- Understanding and harnessing unreduced gametes for crop improvement.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Review
- The Control of the Crossover Localization in Allium.International journal of molecular sciences · 2023Article
- Article
- Distal Bias of Meiotic Crossovers in Hexaploid Bread Wheat Reflects Spatio-Temporal Asymmetry of the Meiotic Program.Frontiers in plant science · 2021Article
- Concurrent Disruption of Genetic Interference and Increase of Genetic Recombination Frequency in Hybrid Rice Using CRISPR/Cas9.Frontiers in plant science · 2021Article
- Dmc1 is a candidate for temperature tolerance during wheat meiosis.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
- Understanding and Manipulating Meiotic Recombination in Plants.Plant physiology · 2017Review
- Cytomixis doesn't induce obvious changes in chromatin modifications and programmed cell death in tobacco male meiocytes.Frontiers in plant science · 2015Article
- The subtelomeric region is important for chromosome recognition and pairing during meiosis.Scientific reports · 2014Article
- The synaptonemal complex protein ZYP1 is required for imposition of meiotic crossovers in barley.The Plant cell · 2014Article
- High resolution analysis of meiotic chromosome structure and behaviour in barley (Hordeum vulgare L.).PloS one · 2012Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe synaptonemal complex (SC) is a proteinaceous tripartite structure used to hold homologous chromosomes together during the early stages of meiosis. The yeast ZIP1 and its homologues in other species have previously been characterised as the transverse filament protein of the synaptonemal complex. Proper installation of ZYP1 along chromosomes has been shown to be dependent on the axial element-associated protein, ASY1 in Arabidopsis.
resultsHere we report the isolation of the wheat (Triticum aestivum) ZYP1 (TaZYP1) and its expression profile (during and post-meiosis) in wild-type, the ph1b deletion mutant as well as in Taasy1 RNAi knock-down mutants. TaZYP1 has a putative DNA-binding S/TPXX motif in its C-terminal region and we provide evidence that TaZYP1 interacts non-preferentially with both single- and double-stranded DNA in vitro. 3-dimensional dual immunofluorescence localisation assays conducted with an antibody raised against TaZYP1 show that TaZYP1 interacts with chromatin during meiosis but does not co-localise to regions of chromatin where TaASY1 is present. The TaZYP1 signal lengthens into regions of chromatin where TaASY1 has been removed in wild-type but this appears delayed in the ph1b mutant. The localisation profile of TaZYP1 in four Taasy1 knock-down mutants is similar to wild-type but TaZYP1 signal intensity appears weaker and more diffused.
conclusionsIn contrast to previous studies performed on plant species where ZYP1 signal is sandwiched by ASY1 signal located on both axial elements of the SC, data from the 3-dimensional dual immunofluorescence localisation assays conducted in this study show that TaZYP1 signal only lengthens into regions of chromatin after TaASY1 signal is being unloaded. However, the observation that TaZYP1 loading appears delayed in both the ph1b and Taasy1 mutants suggests that TaASY1 may still be essential for TaZYP1 to play a role in SC formation during meiosis. These data further suggest that the temporal installation of ZYP1 onto pairing homologous chromosomes in wheat is different to that of other plant species and highlights the need to study this synaptonemal complex protein on a species to species basis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.