ArticleVirology journal2012
Rapid detection of sacbrood virus (SBV) by one-step reverse transcription loop-mediated isothermal amplification assay.
Article in Virology journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
4 citing papers in PubMed, 7 citations in OpenAlex.
- Development of a Kit for Rapid Immunochromatographic Detection of Sacbrood Virus InfectingViruses · 2021Article
- Updating Sacbrood Virus Quantification PCR Method Using a TaqMan-MGB Probe.Veterinary sciences · 2021Article
- Current Status of Loop-Mediated Isothermal Amplification Technologies for the Detection of Honey Bee Pathogens.Frontiers in veterinary science · 2021Review
- Colorimetric biosensors for point-of-care virus detections.Materials science for energy technologies · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSacbrood virus (SBV) primarily infects honeybee broods, and in order to deal with the problem cost effective detection methods are required.
findingsA one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid identification of SBV. The data demonstrated that, in a simple water bath, SBV RNA could be detected as early as 20 min at 65°C, and a positive amplification reaction was visible to the naked eye due to a color change brought on by the addition of nucleic acid stain SYBR Green.
conclusionsThe current study presents a method for the rapid and simple detection of SBV by RT-LAMP with high sensitivity and analytic specificity.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.