ArticleBiophysical journal2012
Kinetics and mechanics of two-dimensional interactions between T cell receptors and different activating ligands.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 73 citations in OpenAlex.
- Mechanotransduction through T cell receptors: consensus, controversies and future outlooks.Experimental & molecular medicine · 2026Review
- Murine T-cell receptor OT-I exhibits imperfect discrimination between foreign and self-antigens.The EMBO journal · 2026Article
- Morphodynamics of T-lymphocytes: Scanning to spreading.Biophysical journal · 2024Review
- Mathematical models of TCR initial triggering.Frontiers in immunology · 2024Review
- A dynamic biomimetic model of the membrane-bound CD4-CD3-TCR complex during pMHC disengagement.Biophysical journal · 2023Article
- Harnessing αβ T cell receptor mechanobiology to achieve the promise of immuno-oncology.Proceedings of the National Academy of Sciences of the United States of America · 2023Review
- Mechanical forces impair antigen discrimination by reducing differences in T-cell receptor/peptide-MHC off-rates.The EMBO journal · 2023Article
- Two-dimensional measurements of receptor-ligand interactions.Frontiers in molecular biosciences · 2023Review
- The perception and response of T cells to a changing environment are based on the law of initial value.Science signaling · 2022Review
- Supported Lipid Bilayers and the Study of Two-Dimensional Binding Kinetics.Frontiers in molecular biosciences · 2022Review
- A computational algorithm to assess the physiochemical determinants of T cell receptor dissociation kinetics.Computational and structural biotechnology journal · 2022Article
- Using molecular dynamics simulations to interrogate T cell receptor non-equilibrium kinetics.Computational and structural biotechnology journal · 2022Article
- Mechanotransduction as a major driver of cell behaviour: mechanisms, and relevance to cell organization and future research.Open biology · 2021Review
- Modelling the interplay between the CD4Journal of mathematical biology · 2021Article
- Controlling T cells spreading, mechanics and activation by micropatterning.Scientific reports · 2021Article
- Article
- Membrane Organization and Physical Regulation of Lymphocyte Antigen Receptors: A Biophysicist's Perspective.The Journal of membrane biology · 2019Review
- TCR-pMHC kinetics under force in a cell-free system show no intrinsic catch bond, but a minimal encounter duration before binding.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Nanobody-CD16 Catch Bond Reveals NK Cell Mechanosensitivity.Biophysical journal · 2019Article
- Biphasic mechanosensitivity of T cell receptor-mediated spreading of lymphocytes.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Adaptive immune responses are driven by interactions between T cell antigen receptors (TCRs) and complexes of peptide antigens (p) bound to Major Histocompatibility Complex proteins (MHC) on the surface of antigen-presenting cells. Many experiments support the hypothesis that T cell response is quantitatively and qualitatively dependent on the so-called strength of TCR/pMHC association. Most available data are correlations between binding parameters measured in solution (three-dimensional) and pMHC activation potency, suggesting that full lymphocyte activation required a minimal lifetime for TCR/pMHC interaction. However, recent reports suggest important discrepancies between the binding properties of ligand-receptor couples measured in solution (three-dimensional) and those measured using surface-bound molecules (two-dimensional). Other reports suggest that bond mechanical strength may be important in addition to kinetic parameters. Here, we used a laminar flow chamber to monitor at the single molecule level the two-dimensional interaction between a recombinant human TCR and eight pMHCs with variable potency. We found that 1), two-dimensional dissociation rates were comparable to three-dimensional parameters previously obtained with the same molecules; 2), no significant correlation was found between association rates and activating potency of pMHCs; 3), bond mechanical strength was partly independent of bond lifetime; and 4), a suitable combination of bond lifetime and bond strength displayed optimal correlation with activation efficiency. These results suggest possible refinements of contemporary models of signal generation by T cell receptors. In conclusion, we reported, for the first time to our knowledge, the two-dimensional binding properties of eight TCR/pMHC couples in a cell-free system with single bond resolution.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.