ArticleBiophysical journal2012
A multiphysics model of in vitro transcription coupling enzymatic reaction and precipitation formation.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- IntegratedMolecular therapy. Nucleic acids · 2026Article
- A Modular Mechanistic In Silico Model for In Vitro Transcription Process Yield and Product Quality Prediction.Biotechnology and bioengineering · 2026Article
- Model-Based Optimization of Fed-Batch In Vitro Transcription.Chembiochem : a European journal of chemical biology · 2025Article
- Design and Optimization of Isothermal Gene Amplification for Generation of High-Gain Oligonucleotide Products by MicroRNAs.ACS measurement science au · 2024Article
- Recombinant T7 RNA polymerase production using ClearColi BL21(DE3) and animal-free media for in vitro transcription.Applied microbiology and biotechnology · 2024Article
- Co-transcriptional production of programmable RNA condensates and synthetic organelles.Nature nanotechnology · 2024Article
- Development of a capillary zone electrophoresis method to monitor magnesium ion consumption during in vitro transcription for mRNA production.Analytical and bioanalytical chemistry · 2024Article
- Digital Twin Fundamentals of mRNAACS omega · 2024Article
- Quality by Digital Design for Developing Platform RNA Vaccine and Therapeutic Manufacturing Processes.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Urea supplementation improves mRNA in vitro transcription by decreasing both shorter and longer RNA byproducts.RNA biology · 2024Article
- Bacteriophage RNA polymerases: catalysts for mRNA vaccines and therapeutics.Frontiers in molecular biosciences · 2024Review
- Purification and characterization of inorganic pyrophosphatase for in vitro RNA transcription.Biochemistry and cell biology = Biochimie et biologie cellulaire · 2022Article
- Quality by design modelling to support rapid RNA vaccine production against emerging infectious diseases.NPJ vaccines · 2021Article
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Authors and funding
3 authors.
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Abstract
Multiphysics modeling, which integrates the models studied in different disciplines so far, is an indispensable approach toward a comprehensive understanding of biological systems composed of diverse phenomena. However, the variety of the models is narrower than the actual diverse phenomena because of the difficulty in coupling independent models separately studied in different disciplines for the actual coupled phenomena. In this study, we develop a mathematical model coupling an enzymatic reaction and mineralization formation. As a test case, we selected an in vitro transcription system where a transcription reaction occurs along with the precipitation formation of magnesium pyrophosphate (Mg(2)PPi). To begin, we experimentally elucidated how the transcription reaction and the precipitation formation are coupled. In the analysis, we applied a Michaelis-Menten-type equation to the transcription reaction and a semiempirical equation describing the correlation between the induction period and the supersaturation ratio to the precipitation formation, respectively. Based on the experimental results, we then integrated these two models. These models were connected by supersaturation that increases as the transcription reaction proceeds and becomes the driving force of the precipitation. We believe that our modeling approach could significantly contribute to the development of newer multiphysics models in systems biology such as bone metabolic networks.
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