ArticleJournal of neurochemistry2012
Cytosolic acidification and intracellular zinc release in hippocampal neurons.
Article in Journal of neurochemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 37 citations in OpenAlex.
- Calcium signaling hypothesis: A non-negligible pathogenesis in Alzheimer's disease.Journal of advanced research · 2025Review
- Metallothionein III Mediates CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- The interplay of intracellular calcium and zinc ions in response to electric field stimulation in primary rat cortical neuronsFrontiers in cellular neuroscience · 2023Article
- Intracellular zinc signaling influences NMDA receptor function by enhancing the interaction of ZnT1 with GluN2A.Neuroscience letters · 2022Article
- Neuronal signalling of zinc: from detection and modulation to function.Open biology · 2022Review
- Zinc Modulation of Neuronal Calcium Sensor Proteins: Three Modes of Interaction with Different Structural Outcomes.Biomolecules · 2022Article
- Expression of SSEA-4 and Oct-4 from somatic cells in primary mouse gastric cell culture induced by brief strong acid.Molecular and cellular biochemistry · 2021Article
- Spontaneous, synchronous zinc spikes oscillate with neural excitability and calcium spikes in primary hippocampal neuron culture.Journal of neurochemistry · 2021Article
- Tools and techniques for illuminating the cell biology of zinc.Biochimica et biophysica acta. Molecular cell research · 2021Review
- The change of intracellular zinc distribution after strong acid challenge.International journal of physiology, pathophysiology and pharmacology · 2021Article
- Dissociated Hippocampal Neurons Exhibit Distinct ZnACS chemical neuroscience · 2020Article
- Elucidating the HInternational journal of molecular sciences · 2020Article
- Intracellular ZnScientific reports · 2019Article
- Lysosomal dysfunction in proteinopathic neurodegenerative disorders: possible therapeutic roles of cAMP and zinc.Molecular brain · 2019Review
- Neurotoxicity Linked to Dysfunctional Metal Ion Homeostasis and Xenobiotic Metal Exposure: Redox Signaling and Oxidative Stress.Antioxidants & redox signaling · 2018Review
- Functional Status of Neuronal Calcium Sensor-1 Is Modulated by Zinc Binding.Frontiers in molecular neuroscience · 2018Article
- The Emerging Role of Zinc in the Pathogenesis of Multiple Sclerosis.International journal of molecular sciences · 2017Review
- Neuronal acid-induced [Zn²⁺]i elevations calibrated using the low-affinity ratiometric probe FuraZin-1.Journal of neurochemistry · 2015Article
- Zinc-permeable ion channels: effects on intracellular zinc dynamics and potential physiological/pathophysiological significance.Current medicinal chemistry · 2015Review
- Influence of zinc on calcium-dependent signal transduction pathways during aluminium-induced neurodegeneration.Molecular neurobiology · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
In neurons exposed to glutamate, Ca²⁺ influx triggers intracellular Zn²⁺ release via an as yet unclear mechanism. As glutamate induces a Ca²⁺-dependent cytosolic acidification, the present work tested the relationships among intracellular Ca²⁺ concentration ([Ca²⁺](i)), intracellular pH (pH(i) ), and [Zn²⁺](i). Cultured hippocampal neurons were exposed to glutamate and glycine (Glu/Gly), while [Zn²⁺](i), [Ca²⁺](i) and pH(i) were monitored using FluoZin-3, Fura2-FF, and 2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein, respectively. Glu/Gly applications decreased pH(i) to 6.1 and induced intracellular Zn²⁺ release in a Ca²⁺-dependent manner, as expected. The pH(i) drop reduced the affinity of FluoZin-3 and Fura-2-FF for Zn²⁺. The rate of Glu/Gly-induced [Zn²⁺](i) increase was not correlated with the rate of [Ca²⁺](i) increase. Instead, the extent of [Zn²⁺](i) elevations corresponded well to the rate of pH(i) drop. Namely, [Zn²⁺](i) increased more in more highly acidified neurons. Inhibiting the mechanisms responsible for the Ca²⁺-dependent pH(i) drop (plasmalemmal Ca²⁺ pump and mitochondria) counteracted the Glu/Gly-induced intracellular Zn²⁺ release. Alkaline pH (8.5) suppressed Glu/Gly-induced intracellular Zn²⁺ release whereas acidic pH (6.0) enhanced it. A pH(i) drop to 6.0 (without any Ca²⁺ influx or glutamate receptor activation) led to intracellular Zn²⁺ release; the released Zn²⁺ (free Zn²⁺ plus Zn²⁺) bound to Fura-2FF and FluoZin-3) reached 1 μM.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.