ArticleRNA biology2012
Deep sequencing uncovers numerous small RNAs on all four replicons of the plant pathogen Agrobacterium tumefaciens.
Article in RNA biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 89 citations in OpenAlex.
- Comparative transcriptomics reveals context- and strain-specific regulatory programmes ofMicrobial genomics · 2025Article
- Methods for Bioinformatic Prediction of Genuine sRNAs from Outer Membrane Vesicles.Methods in molecular biology (Clifton, N.J.) · 2024Article
- The Role of Iron in Phytopathogenic Microbe-Plant Interactions: Insights into Virulence and Host Immune Response.Plants (Basel, Switzerland) · 2023Review
- Article
- Do small RNAs unlock the below ground microbiome-plant interaction mystery?Frontiers in molecular biosciences · 2022Review
- Motility control through an anti-activation mechanism in Agrobacterium tumefaciens.Molecular microbiology · 2021Article
- Arginine-Rich Small Proteins with a Domain of Unknown Function, DUF1127, Play a Role in Phosphate and Carbon Metabolism of Agrobacterium tumefaciens.Journal of bacteriology · 2020Article
- Interplay between Regulatory RNAs and Signal Transduction Systems during Bacterial Infection.Genes · 2020Review
- Whole-genome Identification of Transcriptional Start Sites by Differential RNA-seq in Bacteria.Bio-protocol · 2020Article
- A central role for the transcriptional regulator VtlR in small RNA-mediated gene regulation in Agrobacterium tumefaciens.Scientific reports · 2020Article
- Structure of a Core Promoter in Bifidobacterium longum NCC2705.Journal of bacteriology · 2020Article
- Transcription attenuation-derived small RNA rnTrpL regulates tryptophan biosynthesis gene expression in trans.Nucleic acids research · 2019Article
- The RNase YbeY Is Vital for Ribosome Maturation, Stress Resistance, and Virulence of the Natural Genetic EngineerJournal of bacteriology · 2019Article
- Article
- Functional Characterization of a Global Virulence Regulator Hfq and Identification of Hfq-Dependent sRNAs in the Plant PathogenFrontiers in microbiology · 2019Article
- Article
- Article
- Host-Pathogen interactions modulated by small RNAs.RNA biology · 2017Review
- Genome-Wide Detection of Small Regulatory RNAs in Deep-Sea BacteriumFrontiers in microbiology · 2017Article
- A Genome-Wide Prediction and Identification of Intergenic Small RNAs by Comparative Analysis inFrontiers in microbiology · 2017Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Agrobacterium species are capable of interkingdom gene transfer between bacteria and plants. The genome of Agrobacterium tumefaciens consists of a circular and a linear chromosome, the At-plasmid and the Ti-plasmid, which harbors bacterial virulence genes required for tumor formation in plants. Little is known about promoter sequences and the small RNA (sRNA) repertoire of this and other α-proteobacteria. We used a differential RNA sequencing (dRNA-seq) approach to map transcriptional start sites of 388 annotated genes and operons. In addition, a total number of 228 sRNAs was revealed from all four Agrobacterium replicons. Twenty-two of these were confirmed by independent RNA gel blot analysis and several sRNAs were differentially expressed in response to growth media, growth phase, temperature or pH. One sRNA from the Ti-plasmid was massively induced under virulence conditions. The presence of 76 cis-antisense sRNAs, two of them on the reverse strand of virulence genes, suggests considerable antisense transcription in Agrobacterium. The information gained from this study provides a valuable reservoir for an in-depth understanding of sRNA-mediated regulation of the complex physiology and infection process of Agrobacterium.
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