ArticleAMB Express2012
Recombinant bromelain production in Escherichia coli: process optimization in shake flask culture by response surface methodology.
Article in AMB Express, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 47 citations in OpenAlex.
- Construction of Pseudomonas aeruginosa SDK-6 with synthetic lipase gene cassette and optimization of different parameters using response surface methodology for over-expression of recombinant lipase.Folia microbiologica · 2024Article
- Integrated bioprocessing and genetic strategies to enhance soluble expression of anti-HER2 immunotoxin in E. Coli.AMB Express · 2024Article
- Indirect optimization of staphylokinase expression level in dicistronic auto-inducible system.AMB Express · 2022Article
- Heterologous Expression and Catalytic Properties of Codon-Optimized Small-Sized Bromelain from MD2 Pineapple.Molecules (Basel, Switzerland) · 2022Article
- Anticancer properties of bromelain: State-of-the-art and recent trends.Frontiers in oncology · 2022Review
- Bromelain a Potential Bioactive Compound: A Comprehensive Overview from a Pharmacological Perspective.Life (Basel, Switzerland) · 2021Review
- Optimization and Formulation of Fucoxanthin-Loaded Microsphere (F-LM) Using Response Surface Methodology (RSM) and Analysis of Its Fucoxanthin Release Profile.Molecules (Basel, Switzerland) · 2019Article
- The Goldilocks Approach: A Review of Employing Design of Experiments in Prokaryotic Recombinant Protein Production.Bioengineering (Basel, Switzerland) · 2018Review
- Optimization of Production Conditions for Protoplasts and Polyethylene Glycol-Mediated Transformation ofMolecules (Basel, Switzerland) · 2018Article
- A minimum information standard for reproducing bench-scale bacterial cell growth and productivity.Communications biology · 2018Article
- Optimization of medium compositions to improve a novel glycoprotein production by Streptomyces kanasenisi ZX01.AMB Express · 2017Article
- Effect of Culture Condition Variables on Human Endostatin Gene Expression inJundishapur journal of microbiology · 2016Article
- Cloning and Expression of Recombinant Human Endostatin in Periplasm of Escherichia coli Expression System.Advanced pharmaceutical bulletin · 2016Article
- Optimization of Recombinant Expression of Synthetic Bacterial Phytase in Pichia pastoris Using Response Surface Methodology.Jundishapur journal of microbiology · 2015Article
- Cultivation Conditions for Phytase Production from Recombinant Escherichia coli DH5α.Microbiology insights · 2013Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bromelain, a cysteine protease with various therapeutic and industrial applications, was expressed in Escherichia coli, BL21-AI clone, under different cultivation conditions (post-induction temperature, L-arabinose concentration and post-induction period). The optimized conditions by response surface methodology using face centered central composite design were 0.2% (w/v) L-arabinose, 8 hr and 25°C. The analysis of variance coupled with larger value of R2 (0.989) showed that the quadratic model used for the prediction was highly significant (p < 0.05). Under the optimized conditions, the model produced bromelain activity of 9.2 U/mg while validation experiments gave bromelain activity of 9.6 ± 0.02 U/mg at 0.15% (w/v) L-arabinose, 8 hr and 27°C. This study had innovatively developed cultivation conditions for better production of recombinant bromelain in shake flask culture.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.