ArticleInternational journal of nanomedicine2012
Influence of charge ratio of liposome/DNA complexes on their size after extrusion and transfection efficiency.
Article in International journal of nanomedicine, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 35 citations in OpenAlex.
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- Optimization of DOTAP/Cholesterol Cationic Lipid Nanoparticles for Nucleic Acid Delivery.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Membrane-Fusing Vehicles for Re-Sensitizing Transporter-Mediated Multiple-Drug Resistance in Cancer.Pharmaceutics · 2024Article
- Influence of the Charge Ratio of Guanine-Quadruplex Structure-Based CpG Oligodeoxynucleotides and Cationic DOTAP Liposomes on Cytokine Induction Profiles.Biomolecules · 2023Article
- A novel method for the development of plasmid DNA-loaded nanoliposomes for cancer gene therapy.Drug delivery and translational research · 2022Article
- Optimization of DOTAP/chol Cationic Lipid Nanoparticles for mRNA, pDNA, and Oligonucleotide Delivery.AAPS PharmSciTech · 2022Article
- Complexation design of cationized gelatin and molecular beacon to visualize intracellular mRNA.PloS one · 2021Article
- Impact of the Charge Ratio on the In Vivo Immunogenicity of Lipoplexes.Pharmaceutical research · 2017Article
- Di-Peptide-Modified Gemini Surfactants as Gene Delivery Vectors: Exploring the Role of the Alkyl Tail in Their Physicochemical Behavior and Biological Activity.The AAPS journal · 2016Article
- Influence of cationic lipid concentration on properties of lipid-polymer hybrid nanospheres for gene delivery.International journal of nanomedicine · 2015Article
- Gene delivery to Her-2+ breast cancer cells using a two-component delivery system to achieve specificity.Nanomedicine : nanotechnology, biology, and medicine · 2014Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPhysicochemical characteristics of liposome/DNA complexes influence transfection efficiency and affect each other in a very intricate way. The result of this is discrepancies in conclusions drawn about the individual influence of each one.
methodsAiming to elucidate the influence of liposome/DNA charge ratio and size on transfection efficiency and on each other, we used liposome/DNA complexes with charge ratio (+/-) in the range of 1-50 and extruded through membranes of 400, 200, and 100 nm. Plasmid DNA encoding green fluorescent protein was used to measure transfection efficiency by flow cytometry. Sizes of liposome/DNA complexes were measured by dynamic light scattering.
resultsLiposome size was reduced after extrusion but this was mainly driven by the charge ratio and not by the size of the membrane pores. Reduction of complex size at each charge ratio positively correlated with transfection efficiency. When the size of the complexes was approximately constant, increasing the charge ratio was found to promote transfection efficiency. Cationic lipid N-(1-(2,3-dioleoyloxy)propyl)N,N,N trimethylammonium chloride was used for modulation of positive charge and a cytotoxicity test showed that increasing its amount increases cytotoxicity.
conclusionIt can be concluded that charge ratio dictates the size of the complex whereas overall size reduction and higher charge ratios promote transfection efficiency in vitro.
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