ArticleThe Journal of biological chemistry2012
Role of N-glycosylation sites and CXC motifs in trafficking of medicago truncatula Nod factor perception protein to plasma membrane.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 58 citations in OpenAlex.
- The receptor-like cytoplasmic kinase AeRLCK2 mediates Nod-independent rhizobial symbiosis in Aeschynomene legumes.The Plant cell · 2025Article
- A plant Lysin Motif Receptor-Like Kinase plays an ancestral function in mycorrhiza.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- Emerging Roles of Receptor-like Protein Kinases in Plant Response to Abiotic Stresses.International journal of molecular sciences · 2023Review
- LysM receptor-like kinases involved in immunity perceive lipo-chitooligosaccharides in mycotrophic plants.Plant physiology · 2023Article
- The grapevine LysM receptor-like kinase VvLYK5-1 recognizes chitin oligomers through its association with VvLYK1-1.Frontiers in plant science · 2023Article
- Nod factor perception: an integrative view of molecular communication during legume symbiosis.Plant molecular biology · 2022Review
- Innovation and appropriation in mycorrhizal and rhizobial Symbioses.The Plant cell · 2022Article
- Spatial Mapping of PlantFrontiers in plant science · 2022Article
- LCO Receptors Involved in Arbuscular Mycorrhiza Are Functional for Rhizobia Perception in Legumes.Current biology : CB · 2019Article
- di-Cysteine motifs in the C-terminus of plant HMA4 proteins confer nanomolar affinity for zinc and are essential for HMA4 function in vivo.Journal of experimental botany · 2018Article
- Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H.Journal of visualized experiments : JoVE · 2018Article
- Evolutionary History of Plant LysM Receptor Proteins Related to Root Endosymbiosis.Frontiers in plant science · 2018Article
- Tomato Prenylated RAB Acceptor Protein 1 Modulates Trafficking and Degradation of the Pattern Recognition Receptor LeEIX2, Affecting the Innate Immune Response.Frontiers in plant science · 2018Article
- LysM Receptor-Like Kinase and LysM Receptor-Like Protein Families: An Update on Phylogeny and Functional Characterization.Frontiers in plant science · 2018Review
- PUB1 Interacts with the Receptor Kinase DMI2 and Negatively Regulates Rhizobial and Arbuscular Mycorrhizal Symbioses through Its Ubiquitination Activity in Medicago truncatula.Plant physiology · 2016Article
- Sequence and expression analysis of putative Arachis hypogaea (peanut) Nod factor perception proteins.Journal of plant research · 2015Article
- Molecular diversity of LysM carbohydrate-binding motifs in fungi.Current genetics · 2015Review
- Site-specific N-glycosylation of the S-locus receptor kinase and its role in the self-incompatibility response of the brassicaceae.The Plant cell · 2014Article
- Molecular basis for bacterial peptidoglycan recognition by LysM domains.Nature communications · 2014Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The lysin motif receptor-like kinase, NFP (Nod factor perception), is a key protein in the legume Medicago truncatula for the perception of lipochitooligosaccharidic Nod factors, which are secreted bacterial signals essential for establishing the nitrogen-fixing legume-rhizobia symbiosis. Predicted structural and genetic analyses strongly suggest that NFP is at least part of a Nod factor receptor, but few data are available about this protein. Characterization of a variant encoded by the mutant allele nfp-2 revealed the sensitivity of this protein to the endoplasmic reticulum quality control mechanisms, affecting its trafficking to the plasma membrane. Further analysis revealed that the extensive N-glycosylation of the protein is not essential for biological activity. In the NFP extracellular region, two CXC motifs and two other Cys residues were found to be involved in disulfide bridges, and these are necessary for correct folding and localization of the protein. Analysis of the intracellular region revealed its importance for biological activity but suggests that it does not rely on kinase activity. This work shows that NFP trafficking to the plasma membrane is highly sensitive to regulation in the endoplasmic reticulum and has identified structural features of the protein, particularly disulfide bridges involving CXC motifs in the extracellular region that are required for its biological function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.