ArticleThe Journal of biological chemistry2012
Determination of protein interactome of transcription factor Sox2 in embryonic stem cells engineered for inducible expression of four reprogramming factors.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 66 citations in OpenAlex.
- Mechanisms coordinating exit from the stem cell state in mammals.Genes & development · 2026Review
- Krüppel-like factor 4 regulates cellular proliferation and differentiation in human bone marrow-derived mesenchymal stem cells.Biochemistry and biophysics reports · 2025Article
- Review
- Loss of H3K9 trimethylation alters chromosome compaction and transcription factor retention during mitosis.Nature structural & molecular biology · 2023Article
- Application of Skyline for Analysis of Protein-Protein Interactions In Vivo.Molecules (Basel, Switzerland) · 2021Article
- Article
- Of numbers and movement - understanding transcription factor pathogenesis by advanced microscopy.Disease models & mechanisms · 2020Review
- Tumor quiescence: elevating SOX2 in diverse tumor cell types downregulates a broad spectrum of the cell cycle machinery and inhibits tumor growth.BMC cancer · 2020Article
- R-loops coordinate with SOX2 in regulating reprogramming to pluripotency.Science advances · 2020Article
- The Sox2 transcription factor binds RNA.Nature communications · 2020Article
- Interaction of Sox2 with RNA binding proteins in mouse embryonic stem cells.Experimental cell research · 2019Article
- KLF4 protein stability regulated by interaction with pluripotency transcription factors overrides transcriptional control.Genes & development · 2019Article
- Differentiation of Stem Cells from Human Exfoliated Deciduous Teeth into Retinal Photoreceptor-Like Cells and Their SustainabilityStem cells international · 2019Article
- "Nutrient-sensing" and self-renewal: O-GlcNAc in a new role.Journal of bioenergetics and biomembranes · 2018Review
- KLF4 Nuclear Export Requires ERK Activation and Initiates Exit from Naive Pluripotency.Stem cell reports · 2018Article
- Identification of the SOX2 Interactome by BioID Reveals EP300 as a Mediator of SOX2-dependent Squamous Differentiation and Lung Squamous Cell Carcinoma Growth.Molecular & cellular proteomics : MCP · 2017Article
- The dark side of SOX2: cancer - a comprehensive overview.Oncotarget · 2017Review
- SMARCD2 subunit of SWI/SNF chromatin-remodeling complexes mediates granulopoiesis through a CEBPɛ dependent mechanism.Nature genetics · 2017Article
- Icaritin enhances mESC self-renewal through upregulating core pluripotency transcription factors mediated by ERα.Scientific reports · 2017Article
- Master regulators in development: Views from the Drosophila retinal determination and mammalian pluripotency gene networks.Developmental biology · 2017Review
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
Abstract
Unbiased proteomic screens provide a powerful tool for defining protein-protein interaction networks. Previous studies employed multidimensional protein identification technology to identify the Sox2-interactome in embryonic stem cells (ESC) undergoing differentiation in response to a small increase in the expression of epitope-tagged Sox2. Thus far the Sox2-interactome in ESC has not been determined. To identify the Sox2-interactome in ESC, we engineered ESC for inducible expression of different combinations of epitope-tagged Sox2 along with Oct4, Klf4, and c-Myc. Epitope-tagged Sox2 was used to circumvent the lack of suitable Sox2 antibodies needed to perform an unbiased proteomic screen of Sox2-associated proteins. Although i-OS-ESC differentiate when both Oct4 and Sox2 are elevated, i-OSKM-ESC do not differentiate even when the levels of the four transcription factors are coordinately elevated ∼2-3-fold. Our findings with i-OS-ESC and i-OSKM-ESC provide new insights into the reasons why ESC undergo differentiation when Sox2 and Oct4 are elevated in ESC. Importantly, the use of i-OSKM-ESC enabled us to identify the Sox2-interactome in undifferentiated ESC. Using multidimensional protein identification technology, we identified >70 proteins that associate with Sox2 in ESC. We extended these findings by testing the function of the Sox2-assoicated protein Smarcd1 and demonstrate that knockdown of Smarcd1 disrupts the self-renewal of ESC and induces their differentiation. Together, our work provides the first description of the Sox2-interactome in ESC and indicates that Sox2 along with other master regulators is part of a highly integrated protein-protein interaction landscape in ESC.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.