ArticleThe Journal of biological chemistry2012
Multiple forms of Spire-actin complexes and their functional consequences.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Docking and molecular dynamics studies of human ezrin protein with a modelled SARS-CoV-2 endodomain and their interaction with potential invasion inhibitors.Journal of King Saud University. Science · 2022Article
- Zero-mode waveguides visualize the first steps during gelsolin-mediated actin filament formation.Biophysical journal · 2022Article
- Spire stimulates nucleation by Cappuccino and binds both ends of actin filaments.Molecular biology of the cell · 2020Article
- Actin Cross-Linking Toxin Is a Universal Inhibitor of Tandem-Organized and Oligomeric G-Actin Binding Proteins.Current biology : CB · 2018Article
- Actin filament assembly by bacterial factors VopL/F: Which end is up?The Journal of cell biology · 2017Article
- Myocardin-Related Transcription Factor A Activation by Competition with WH2 Domain Proteins for Actin Binding.Molecular and cellular biology · 2016Article
- Metavinculin Tunes the Flexibility and the Architecture of Vinculin-Induced Bundles of Actin Filaments.Journal of molecular biology · 2015Article
- Structure of a Bud6/Actin Complex Reveals a Novel WH2-like Actin Monomer Recruitment Motif.Structure (London, England : 1993) · 2015Article
- Filament assembly by Spire: key residues and concerted actin binding.Journal of molecular biology · 2015Article
- Dendrite architecture organized by transcriptional control of the F-actin nucleator Spire.Development (Cambridge, England) · 2014Article
- Direct interaction between two actin nucleators is required in Drosophila oogenesis.Development (Cambridge, England) · 2013Article
- Structural basis of actin filament nucleation by tandem W domains.Cell reports · 2013Article
- In vitro studies of actin filament and network dynamics.Current opinion in cell biology · 2013Review
- Exploring the possible role of lysine acetylation on Entamoeba histolytica virulence: a focus on the dynamics of the actin cytoskeleton.BioMed research international · 2013Article
- Structure of the formin-interaction domain of the actin nucleation-promoting factor Bud6.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Autoinhibition of the formin Cappuccino in the absence of canonical autoinhibitory domains.Molecular biology of the cell · 2012Article
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5 authors.
Funding
Abstract
Spire is a WH2 domain-containing actin nucleator essential for establishing an actin mesh during oogenesis. In vitro, in addition to nucleating filaments, Spire can sever them and sequester actin monomers. Understanding how Spire is capable of these disparate functions and which are physiologically relevant is an important goal. To study severing, we examined the effect of Drosophila Spire on preformed filaments in bulk and single filament assays. We observed rapid depolymerization of actin filaments by Spire, which we conclude is largely due to its sequestration activity and enhanced by its weak severing activity. We also studied the solution and crystal structures of Spire-actin complexes. We find structural and functional differences between constructs containing four WH2 domains (Spir-ABCD) and two WH2 domains (Spir-CD) that may provide insight into the mechanisms of nucleation and sequestration. Intriguingly, we observed lateral interactions between actin monomers associated with Spir-ABCD, suggesting that the structures built by these four tandem WH2 domains are more complex than originally imagined. Finally, we propose that Spire-actin mixtures contain both nuclei and sequestration structures.
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