ArticleThe Journal of biological chemistry2012
Phosphorylation of ORC2 protein dissociates origin recognition complex from chromatin and replication origins.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 49 citations in OpenAlex.
- DivergentLife science alliance · 2025Article
- Integrative bioinformatics analysis reveals STAT1, ORC2, and GTF2B as critical biomarkers in lupus nephritis with Monkeypox virus infection.Scientific reports · 2025Article
- High resolution profiling of cell cycle-dependent protein and phosphorylation abundance changes in non-transformed cells.Nature communications · 2025Article
- Phosphorylation of Orc6 During Mitosis Regulates DNA Replication and Ribosome Biogenesis.Molecular and cellular biology · 2024Article
- Domain architecture and protein-protein interactions regulate KDM5A recruitment to the chromatin.Epigenetics · 2023Review
- Molecular determinants of phase separation foreLife · 2021Article
- Structural insight into the assembly and conformational activation of human origin recognition complex.Cell discovery · 2020Article
- The E3 ligase RFWD3 stabilizes ORC in a p53-dependent manner.Cell cycle (Georgetown, Tex.) · 2020Article
- Preparation for DNA replication: the key to a successful S phase.FEBS letters · 2019Review
- A new class of disordered elements controls DNA replication through initiator self-assembly.eLife · 2019Article
- Spatial and temporal resolution of mORC4 fluorescent variants reveals structural requirements for achieving higher order self-association and pronuclei entry.Methods and applications in fluorescence · 2019Article
- Cyclin-dependent kinase 1 (CDK1) and CDK2 have opposing roles in regulating interactions of splicing factor 3B1 with chromatin.The Journal of biological chemistry · 2018Article
- Reversible regulation of ORC2 SUMOylation by PIAS4 and SENP2.Oncotarget · 2017Article
- Review
- Structural prediction of the interaction of the tumor suppressor p27BMC bioinformatics · 2017Article
- Roles of SUMO in Replication Initiation, Progression, and Termination.Advances in experimental medicine and biology · 2017Review
- The Replicative Consequences of Papillomavirus E2 Protein Binding to the Origin Replication Factor ORC2.PLoS pathogens · 2016Article
- Regulation of DNA replication proteins in parasitic protozoans: possible role of CDK-like kinases.Current genetics · 2016Review
- SCF(Cyclin F)-dependent degradation of CDC6 suppresses DNA re-replication.Nature communications · 2016Article
- ORC proteins in the mammalian zygote.Cell and tissue research · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During the late M to the G(1) phase of the cell cycle, the origin recognition complex (ORC) binds to the replication origin, leading to the assembly of the prereplicative complex for subsequent initiation of eukaryotic chromosome replication. We found that the cell cycle-dependent phosphorylation of human ORC2, one of the six subunits of ORC, dissociates ORC2, -3, -4, and -5 (ORC2-5) subunits from chromatin and replication origins. Phosphorylation at Thr-116 and Thr-226 of ORC2 occurs by cyclin-dependent kinase during the S phase and is maintained until the M phase. Phosphorylation of ORC2 at Thr-116 and Thr-226 dissociated the ORC2-5 from chromatin. Consistent with this, the phosphomimetic ORC2 protein exhibited defective binding to replication origins as well as to chromatin, whereas the phosphodefective protein persisted in binding throughout the cell cycle. These results suggest that the phosphorylation of ORC2 dissociates ORC from chromatin and replication origins and inhibits binding of ORC to newly replicated DNA.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.