ArticleCytometry. Part B, Clinical cytometry2012
Functional pathway analysis in acute myeloid leukemia using single cell network profiling assay: effect of specimen source (bone marrow or peripheral blood) on assay readouts.
Article in Cytometry. Part B, Clinical cytometry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- Baseline T cell dysfunction by single cell network profiling in metastatic breast cancer patients.Journal for immunotherapy of cancer · 2019Trial
- Development and validation of a single-cell network profiling assay-based classifier to predict response to induction therapy in paediatric patients with de novo acute myeloid leukaemia: a report from the Children's Oncology Group.British journal of haematology · 2013Trial
- Diminished cytokine-induced Jak/STAT signaling is associated with rheumatoid arthritis and disease activity.PloS one · 2021Article
- IRAK4 kinase activity controls Toll-like receptor-induced inflammation through the transcription factor IRF5 in primary human monocytes.The Journal of biological chemistry · 2017Article
- Validation of biomarkers to predict response to immunotherapy in cancer: Volume I - pre-analytical and analytical validation.Journal for immunotherapy of cancer · 2016Review
- Global Cell Proteome Profiling, Phospho-signaling and Quantitative Proteomics for Identification of New Biomarkers in Acute Myeloid Leukemia Patients.Current pharmaceutical biotechnology · 2016Review
- Cell signaling-based classifier predicts response to induction therapy in elderly patients with acute myeloid leukemia.PloS one · 2015Article
- Immune monitoring technology primer: Single Cell Network Profiling (SCNP).Journal for immunotherapy of cancer · 2015Article
- Age-related changes of healthy bone marrow cell signaling in response to growth factors provide insight into low risk MDS.Cytometry. Part B, Clinical cytometry · 2014Article
- Quantitative measurement of alterations in DNA damage repair (DDR) pathways using single cell network profiling (SCNP).Journal of translational medicine · 2014Article
- Future perspectives in melanoma research. Meeting report from the "Melanoma Bridge. Napoli, December 2nd-4th 2012".Journal of translational medicine · 2013Article
- Association between B-cell receptor responsiveness and disease progression in B-cell chronic lymphocytic leukemia: results from single cell network profiling studies.Haematologica · 2013Article
- Article
- AKT signaling as a novel factor associated with in vitro resistance of human AML to gemtuzumab ozogamicin.PloS one · 2013Article
- From single cells to deep phenotypes in cancer.Nature biotechnology · 2012Review
- Using bioinformatic approaches to identify pathways targeted by human leukemogens.International journal of environmental research and public health · 2012Article
- Racial differences in B cell receptor signaling pathway activation.Journal of translational medicine · 2012Article
Corrections and comments
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Authors and funding
12 authors at 5 institutions in 1 country.
Funding
Abstract
backgroundSingle cell network profiling (SCNP) is used to simultaneously measure the effects of modulators on signaling networks at the single cell level. SCNP-based biomarker assays predictive of response to induction therapy and relapse risk in acute myeloid leukemia (AML) patients are being developed. Such assays have typically used bone marrow (BM) as the sample source of blasts. Because circulating peripheral blasts are detectable in ∼65% of AML patients and peripheral blood (PB) sampling is less invasive than BM sampling, this study was performed to assess the effect of sample source on AML blasts signaling as measured in SCNP assay.
methodsSCNP using multiparametric flow cytometry was used to evaluate the activation state of intracellular signaling molecules in leukemic blasts under basal conditions and after treatment with modulators in 46 pairs of BM mononuclear cells/PB mononuclear cells. The relationship between readouts of modulated intracellular proteins ("nodes") was measured using linear regression, Bland-Altman method, and Lin's concordance correlation coefficient.
resultsThe majority (156/161) of signaling nodes show strong correlations between paired PB and BM samples independently from the statistical method used. Notable exceptions were two PB samples with almost undetectable levels of circulating blasts compared with paired BM samples.
conclusionsOur results demonstrate that specimen source (BM or PB) does not significantly affect proteomic signaling in patients with AML and circulating blasts. The ability to use PB as a sample source will facilitate the monitoring of cellular signaling effects following administration of targeted therapies and at time points when BM aspirates are not clinically justifiable.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.