ArticleProceedings of the National Academy of Sciences of the United States of America2012
Solute carrier family 3 member 2 (Slc3a2) controls yolk syncytial layer (YSL) formation by regulating microtubule networks in the zebrafish embryo.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 51 citations in OpenAlex.
- ZebrafishInternational journal of molecular sciences · 2026Article
- Hoxb genes determine the timing of cell ingression by regulating cell surface fluctuations during zebrafish gastrulation.Development (Cambridge, England) · 2025Article
- Ethanol downregulates gastrula gene expression and cell movement, causing symptoms of foetal alcohol spectrum disorders.Biology open · 2025Article
- Origin, form and function of extraembryonic structures in teleost fishes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- Tension-dependent stabilization of E-cadherin limits cell-cell contact expansion in zebrafish germ-layer progenitor cells.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Trophoblast Differentiation Affects Crucial Nutritive Functions of Placental Membrane Transporters.Frontiers in cell and developmental biology · 2022Article
- The Ectodomains of rBAT and 4F2hc Are Fake or Orphan α-Glucosidases.Molecules (Basel, Switzerland) · 2021Review
- The zebrafish cationic amino acid transporter/glycoprotein-associated family: sequence and spatiotemporal distribution during development of the transport system bFish physiology and biochemistry · 2021Article
- Heteromeric Solute Carriers: Function, Structure, Pathology and Pharmacology.Advances in experimental medicine and biology · 2021Review
- Interactions of Environmental Chemicals and Natural Products With ABC and SLC Transporters in the Digestive System of Aquatic Organisms.Frontiers in physiology · 2021Article
- Zebrafish as a Model for Fetal Alcohol Spectrum Disorders.Frontiers in pharmacology · 2021Review
- Review
- Development of the electric organ in embryos and larvae of the knifefish, Brachyhypopomus gauderio.Developmental biology · 2020Article
- Article
- Zebrafish microRNA miR-210-5p inhibits primitive myelopoiesis by silencingThe Journal of biological chemistry · 2019Article
- The peptide transporter 1a of the zebrafishGenes & nutrition · 2019Article
- The primary role of zebrafishDevelopment (Cambridge, England) · 2018Article
- Ikk2 regulates cytokinesis during vertebrate development.Scientific reports · 2017Article
- Extra-embryonic tissue spreading directs early embryo morphogenesis in killifish.Nature communications · 2017Article
- The Physical Basis of Coordinated Tissue Spreading in Zebrafish Gastrulation.Developmental cell · 2017Article
Corrections and comments
- Erratum issuedFaroq, Sumera [corrected to Farooq, Sumera]
Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
The yolk syncytial layer (YSL) in the zebrafish embryo is a multinucleated syncytium essential for embryo development, but the molecular mechanisms underlying YSL formation remain largely unknown. Here we show that zebrafish solute carrier family 3 member 2 (Slc3a2) is expressed specifically in the YSL and that slc3a2 knockdown causes severe YSL defects including clustering of the yolk syncytial nuclei and enhanced cell fusion, accompanied by disruption of microtubule networks. Expression of a constitutively active RhoA mimics the YSL phenotypes caused by slc3a2 knockdown, whereas attenuation of RhoA or ROCK activity rescues the slc3a2-knockdown phenotypes. Furthermore, slc3a2 knockdown significantly reduces tyrosine phosphorylation of c-Src, and overexpression of a constitutively active Src restores the slc3a2-knockdown phenotypes. Our data demonstrate a signaling pathway regulating YSL formation in which Slc3a2 inhibits the RhoA/ROCK pathway via phosphorylation of c-Src to modulate YSL microtubule dynamics. This work illuminates processes at a very early stage of zebrafish embryogenesis and more generally informs the mechanism of cell dynamics during syncytium formation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.