ArticleMolecular & cellular proteomics : MCP2012
Heterogenous turnover of sperm and seminal vesicle proteins in the mouse revealed by dynamic metabolic labeling.
Article in Molecular & cellular proteomics : MCP, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 42 citations in OpenAlex.
- Environmental influences on seminal plasma: Molecular and functional insights.The Journal of reproduction and development · 2026Review
- Variations in conventional and non-conventional semen characteristics of selected rabbit breeds.Animal biotechnology · 2025Article
- On the Role of Seminal Fluid Protein and Nucleic Acid Content in Paternal Epigenetic Inheritance.International journal of molecular sciences · 2022Review
- Sperm acquire epididymis-derived proteins through epididymosomes.Human reproduction (Oxford, England) · 2022Article
- Transcriptomic analysis of the seminal vesicle response to the reproductive toxicant acrylamide.BMC genomics · 2021Article
- Disruption of paternal circadian rhythm affects metabolic health in male offspring via nongerm cell factors.Science advances · 2021Article
- Proteostasis in the Male and Female Germline: A New Outlook on the Maintenance of Reproductive Health.Frontiers in cell and developmental biology · 2021Review
- Seminal fluid and accessory male investment in sperm competition.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Review
- Social status and ejaculate composition in the house mouse.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Article
- Revealing mechanisms of mating plug function under sexual selection.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- A mass spectrometry workflow for measuring protein turnover rates in vivo.Nature protocols · 2019Article
- The remarkable legacy of a father's diet on the health of his offspring.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Protein turnover measurement using selected reaction monitoring-mass spectrometry (SRM-MS).Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2016Article
- Proteome Dynamics: Tissue Variation in the Kinetics of Proteostasis in Intact Animals.Molecular & cellular proteomics : MCP · 2016Article
- Sperm competition risk drives plasticity in seminal fluid composition.BMC biology · 2015Article
- Feasibility of protein turnover studies in prototroph Saccharomyces cerevisiae strains.Analytical chemistry · 2015Article
- Protein kinetic signatures of the remodeling heart following isoproterenol stimulation.The Journal of clinical investigation · 2014Article
- The seminal symphony: how to compose an ejaculate.Trends in ecology & evolution · 2013Article
- Identification and function of proteolysis regulators in seminal fluid.Molecular reproduction and development · 2013Review
- Proteome dynamics: revisiting turnover with a global perspective.Molecular & cellular proteomics : MCP · 2012Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Plasticity in ejaculate composition is predicted as an adaptive response to the evolutionary selective pressure of sperm competition. However, to respond rapidly to local competitive conditions requires dynamic modulation in the production of functionally relevant ejaculate proteins. Here we combine metabolic labeling of proteins with proteomics to explore the opportunity for such modulation within mammalian ejaculates. We assessed the rate at which proteins are synthesized and incorporated in the seminal vesicles of male house mice (Mus musculus domesticus), where major seminal fluid proteins with potential roles in sperm competition are produced. We compared rates of protein turnover in the seminal vesicle with those during spermatogenesis, the timing of which is well known in mice. The subjects were fed a diet containing deuterated valine ([(2)H(8)]valine) for up to 35 days, and the incorporation of dietary-labeled amino acid into seminal vesicle- or sperm-specific proteins was assessed by liquid chromatography-mass spectrometry of samples recovered from the seminal vesicle lumen and cauda epididymis, respectively. Analyses of epididymal contents were consistent with the known duration of spermatogenesis and sperm maturation in this species and in addition revealed evidence for a subset of epididymal proteins subject to rapid turnover. For seminal vesicle proteins, incorporation of the stable isotope was evident from day 2 of labeling, reaching a plateau of labeling by day 24. Hence, even in the absence of copulation, the seminal vesicle proteins and certain epididymal proteins demonstrate considerable turnover, a response that is consonant with the capacity to rapidly modulate protein production. These techniques can now be used to assess the extent of phenotypic plasticity in mammalian ejaculate production and allocation according to social and environmental cues of sperm competition.
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Registered trials
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