ArticleJournal of cell science2012
Epigenetic engineering: histone H3K9 acetylation is compatible with kinetochore structure and function.
Article in Journal of cell science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
76 citing papers in PubMed, 114 citations in OpenAlex.
- Dbp7 interacts with RNA exosome component Dis3 to mediate CENP-A loading to centromeres.Genome biology · 2026Article
- Augmented CENH3 loading is accompanied by transcriptional and epigenetic reprogramming at rice centromeres during meiosis.Genome biology · 2026Article
- Keeping it centered: decoding the activities that regulate yeast histone H3 variant Cse4 and confine it to centromeres.Frontiers in cell and developmental biology · 2026Review
- Polo-like kinase 1 maintains transcription and chromosomal accessibility during mitosis.Molecular biology of the cell · 2025Article
- Determinants of minor satellite RNA function in chromosome segregation in mouse embryonic stem cells.The Journal of cell biology · 2024Article
- The Interaction of NF-κB Transcription Factor with Centromeric Chromatin.The journal of physical chemistry. B · 2024Article
- Centromeric and pericentric transcription and transcripts: their intricate relationships, regulation, and functions.Chromosoma · 2023Review
- Prenatal Stress Induces Long-Term Behavioral Sex-Dependent Changes in Rats Offspring: the Role of the HPA Axis and Epigenetics.Molecular neurobiology · 2023Article
- Actively transcribed rDNA and distal junction (DJ) sequence are involved in association of NORs with nucleoli.Cellular and molecular life sciences : CMLS · 2023Article
- Human centromere repositioning activates transcription and opens chromatin fibre structure.Nature communications · 2022Article
- The Mis6 inner kinetochore subcomplex maintains CENP-A nucleosomes against centromeric non-coding transcription during mitosis.Communications biology · 2022Article
- A transcriptional roadblock protects yeast centromeres.Nucleic acids research · 2022Article
- Article
- CENP-A: A Histone H3 Variant with Key Roles in Centromere Architecture in Healthy and Diseased States.Results and problems in cell differentiation · 2022Article
- Ccp1-Ndc80 switch at the N terminus of CENP-T regulates kinetochore assembly.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Recent insights into mechanisms preventing ectopic centromere formation.Open biology · 2021Review
- Kinetochore assembly throughout the cell cycle.Seminars in cell & developmental biology · 2021Article
- Analysis of Complex DNA Rearrangements during Early Stages of HAC Formation.ACS synthetic biology · 2020Article
- Article
- ZFAT binds to centromeres to control noncoding RNA transcription through the KAT2B-H4K8ac-BRD4 axis.Nucleic acids research · 2020Article
16 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 6 institutions in 3 countries.
Funding
Abstract
Human kinetochores are transcriptionally active, producing very low levels of transcripts of the underlying alpha-satellite DNA. However, it is not known whether kinetochores can tolerate acetylated chromatin and the levels of transcription that are characteristic of housekeeping genes, or whether kinetochore-associated 'centrochromatin', despite being transcribed at a low level, is essentially a form of repressive chromatin. Here, we have engineered two types of acetylated chromatin within the centromere of a synthetic human artificial chromosome. Tethering a minimal NF-κB p65 activation domain within kinetochore-associated chromatin produced chromatin with high levels of histone H3 acetylated on lysine 9 (H3K9ac) and an ~10-fold elevation in transcript levels, but had no substantial effect on kinetochore assembly or function. By contrast, tethering the herpes virus VP16 activation domain produced similar modifications in the chromatin but resulted in an ~150-fold elevation in transcripts, approaching the level of transcription of an endogenous housekeeping gene. This rapidly inactivated kinetochores, causing a loss of assembled CENP-A and blocking further CENP-A assembly. Our data reveal that functional centromeres in vivo show a remarkable plasticity--kinetochores tolerate profound changes to their chromatin environment, but appear to be critically sensitive to the level of centromeric transcription.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.