ArticleJournal of cell science2012
Mammalian erythroblast enucleation requires PI3K-dependent cell polarization.
Article in Journal of cell science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
36 citing papers in PubMed, 57 citations in OpenAlex.
- CDK9 interacts with a RanGTP-importin-β complex to regulate erythroid enucleation.Journal of cell science · 2026Article
- A novel isoform of tensin-1 promotes actin filament assembly for efficient erythroblast enucleation.Blood advances · 2025Article
- Large-scale proteomic and phosphoproteomic analysis of erythroid enucleation and maturation.Blood science (Baltimore, Md.) · 2025Article
- The oncogene protein kinase PIM1 regulates mammalian erythroblast enucleation.Communications biology · 2025Article
- New insights into the mechanisms of red blood cell enucleation: From basics to clinical applications.EJHaem · 2024Review
- A novel role of AURKA kinase in erythroblast enucleation.Haematologica · 2024Article
- Erythroblast enucleation at a glance.Journal of cell science · 2024Review
- PROTAC-mediated vimentin degradation promotes terminal erythroid differentiation of pluripotent stem cells.Stem cell research & therapy · 2024Article
- The impact of erythroblast enucleation efficiency on the severity of anemia in patients with myelodysplastic syndrome.Cell communication and signaling : CCS · 2023Article
- Article
- EHBP1L1, an apicobasal polarity regulator, is critical for nuclear polarization during enucleation of erythroblasts.Blood advances · 2023Article
- Induction of enucleation in primary and immortalized erythroid cells.International journal of hematology · 2022Review
- Centrosome function is critical during terminal erythroid differentiation.The EMBO journal · 2022Article
- Human erythroid differentiation requires VDAC1-mediated mitochondrial clearance.Haematologica · 2022Article
- Altered CaCells · 2021Article
- Vesicular formation regulated by ERK/MAPK pathway mediates human erythroblast enucleation.Blood advances · 2021Article
- Isolation and characterization of fetal nucleated red blood cells from maternal blood as a target for single cell sequencing-based non-invasive genetic testing.Reproductive medicine and biology · 2021Article
- Erythrocytes 3D genome organization in vertebrates.Scientific reports · 2021Article
- Human erythrocytes: cytoskeleton and its origin.Cellular and molecular life sciences : CMLS · 2020Review
- Cellular dynamics of mammalian red blood cell production in the erythroblastic island niche.Biophysical reviews · 2019Review
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
Enucleation, the final step in terminal differentiation of mammalian red blood cells, is an essential process in which the nucleus surrounded by the plasma membrane is budded off from the erythroblast to form a reticulocyte. Most molecular events in enucleation remain unclear. Here we show that enucleation requires establishment of cell polarization that is regulated by the microtubule-dependent local activation of phosphoinositide 3-kinase (PI3K). When the nucleus becomes displaced to one side of the cell, actin becomes restricted to the other side, where dynamic cytoplasmic contractions generate pressure that pushes the viscoelastic nucleus through a narrow constriction in the cell surface, forming a bud. The PI3K products PtdIns(3,4)P₂ and PtdIns(3,4,5)P₃ are highly localized at the cytoplasmic side of the plasma membrane. PI3K inhibition caused impaired cell polarization, leading to a severe delay in enucleation. Depolymerization of microtubules reduced PI3K activity, resulting in impaired cell polarization and enucleation. We propose that enucleation is regulated by microtubules and PI3K signaling in a manner mechanistically similar to directed cell locomotion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.