ArticleJournal of cell science2012
Epithelial organization and cyst lumen expansion require efficient Sec13-Sec31-driven secretion.
Article in Journal of cell science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 25 citations in OpenAlex.
- Review
- The biosynthetic-secretory pathway, supplemented by recycling routes, determines epithelial membrane polarity.Science advances · 2023Article
- Cargo selection in endoplasmic reticulum-to-Golgi transport and relevant diseases.The Journal of clinical investigation · 2023Review
- Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.The Journal of clinical investigation · 2022Article
- Visualization of Procollagen IV Reveals ER-to-Golgi Transport by ERGIC-independent Carriers.Cell structure and function · 2020Article
- ER-to-Golgi trafficking of procollagen in the absence of large carriers.The Journal of cell biology · 2019Article
- Biophysical Control of Bile Duct Epithelial Morphogenesis in Natural and Synthetic Scaffolds.Frontiers in bioengineering and biotechnology · 2019Article
- Models of Intracellular Transport: Pros and Cons.Frontiers in cell and developmental biology · 2019Article
- COPII-dependent ER export in animal cells: adaptation and control for diverse cargo.Histochemistry and cell biology · 2018Review
- Review
- Integrins and epithelial cell polarity.Journal of cell science · 2014Review
- The nuclear pore complex function of Sec13 protein is required for cell survival during retinal development.The Journal of biological chemistry · 2014Article
- In vivo cell biology in zebrafish - providing insights into vertebrate development and disease.Journal of cell science · 2014Review
- Trafficking mechanisms of extracellular matrix macromolecules: insights from vertebrate development and human diseases.The international journal of biochemistry & cell biology · 2014Review
- Early stages of retinal development depend on Sec13 function.Biology open · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Epithelial morphogenesis is directed by interactions with the underlying extracellular matrix. Secretion of collagen and other matrix components requires efficient coat complex II (COPII) vesicle formation at the endoplasmic reticulum. Here, we show that suppression of the outer layer COPII component, Sec13, in zebrafish embryos results in a disorganized gut epithelium. In human intestinal epithelial cells (Caco-2), Sec13 depletion causes defective epithelial polarity and organization on permeable supports. Defects are seen in the ability of cells to adhere to the substrate, form a monolayer and form intercellular junctions. When embedded in a three-dimensional matrix, Sec13-depleted Caco-2 cells form cysts but, unlike controls, are defective in lumen expansion. Incorporation of primary fibroblasts within the three-dimensional culture substantially restores normal morphogenesis. We conclude that efficient COPII-dependent secretion, notably assembly of Sec13-Sec31, is required to drive epithelial morphogenesis in both two- and three-dimensional cultures in vitro, as well as in vivo. Our results provide insight into the role of COPII in epithelial morphogenesis and have implications for the interpretation of epithelial polarity and organization assays in cell culture.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.