ArticleThe Journal of general physiology2012
Properties of Ca2+ sparks revealed by four-dimensional confocal imaging of cardiac muscle.
Article in The Journal of general physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Blink nadir measurements of sarcoplasmic reticulum are consistent with strong local CaBiophysical journal · 2025Article
- Electrophysical cardiac remodeling at the molecular level: Insights into ryanodine receptor activation and calcium-induced calcium release from a stochastic explicit-particle model.Biophysical journal · 2024Article
- Quantification of Sarcoplasmic Reticulum CaCurrent protocols · 2024Article
- High-speed 2D light-sheet fluorescence microscopy enables quantification of spatially varying calcium dynamics in ventricular cardiomyocytes.Frontiers in physiology · 2023Article
- The Functional Characterization of GCaMP3.0 Variants Specifically Targeted to Subcellular Domains.International journal of molecular sciences · 2022Article
- The spatio-temporal properties of calcium transients in hippocampal pyramidal neuronsFrontiers in cellular neuroscience · 2022Article
- 3D dSTORM imaging reveals novel detail of ryanodine receptor localization in rat cardiac myocytes.The Journal of physiology · 2019Article
- CellSpecks: A Software for Automated Detection and Analysis of Calcium Channels in Live Cells.Biophysical journal · 2018Article
- Ryanodine receptor dispersion disrupts CaeLife · 2018Article
- Hierarchical statistical techniques are necessary to draw reliable conclusions from analysis of isolated cardiomyocyte studies.Cardiovascular research · 2017Article
- High speed sCMOS-based oblique plane microscopy applied to the study of calcium dynamics in cardiac myocytes.Journal of biophotonics · 2016Article
- Ryanodine receptor cluster fragmentation and redistribution in persistent atrial fibrillation enhance calcium release.Cardiovascular research · 2015Article
- Imaging fast calcium currents beyond the limitations of electrode techniques.Biophysical journal · 2014Article
- An algorithm for automated detection, localization and measurement of local calcium signals from camera-based imaging.Cell calcium · 2014Article
- Automated detection and analysis of Ca(2+) sparks in x-y image stacks using a thresholding algorithm implemented within the open-source image analysis platform ImageJ.Biophysical journal · 2014Article
- Life and death of a cardiac calcium spark.The Journal of general physiology · 2013Article
- Ca²⁺ waves in the heart.Journal of molecular and cellular cardiology · 2013Review
- Bradycardia alters Ca(2+) dynamics enhancing dispersion of repolarization and arrhythmia risk.American journal of physiology. Heart and circulatory physiology · 2013Article
- Using two dyes with the same fluorophore to monitor cellular calcium concentration in an extended range.PloS one · 2013Article
- Ca(2+) release events in cardiac myocytes up close: insights from fast confocal imaging.PloS one · 2013Article
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Authors and funding
3 authors.
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Abstract
Parameters (amplitude, width, kinetics) of Ca(2+) sparks imaged confocally are affected by errors when the spark source is not in focus. To identify sparks that were in focus, we used fast scanning (LSM 5 LIVE; Carl Zeiss) combined with fast piezoelectric focusing to acquire x-y images in three planes at 1-µm separation (x-y-z-t mode). In 3,000 x-y scans in each of 34 membrane-permeabilized cat atrial cardiomyocytes, 6,906 sparks were detected. 767 sparks were in focus. They had greater amplitude, but their spatial width and rise time were similar compared with all sparks recorded. Their distribution of amplitudes had a mode at ΔF/F(0) = 0.7. The Ca(2+) release current underlying in-focus sparks was 11 pA, requiring 20 to 30 open channels, a number at the high end of earlier estimates. Spark frequency was greater than in earlier imaging studies of permeabilized ventricular cells, suggesting a greater susceptibility to excitation, which could have functional relevance for atrial cells. Ca(2+) release flux peaked earlier than the time of peak fluorescence and then decayed, consistent with significant sarcoplasmic reticulum (SR) depletion. The evolution of fluorescence and release flux were strikingly similar for in-focus sparks of different rise time (T). Spark termination involves both depletion of Ca(2+) in the SR and channel closure, which may be synchronized by depletion. The observation of similar flux in sparks of different T requires either that channel closure and other termination processes be independent of the determinants of flux (including [Ca(2+)](SR)) or that different channel clusters respond to [Ca(2+)](SR) with different sensitivity.
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