ArticleJournal of the American Association for Laboratory Animal Science : JAALAS2011
Comparison of traditional and PCR methods during screening for and confirmation of Aspiculuris tetraptera in a mouse facility.
Article in Journal of the American Association for Laboratory Animal Science : JAALAS, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 23 citations in OpenAlex.
- Differential Effect of Ivermectin on Intestinal Helminths in Multimmate Mouse (Mastomys natalensis).Veterinary medicine and science · 2026Article
- A survey on the implementation of environmental health monitoring in mouse facilities in German-speaking countries.PloS one · 2025Article
- Comparison of Rodent Infectious Agent Detection by Exhaust Dust Testing and Traditional Sentinel Testing Using Quantitative Polymerase Chain Reaction.Journal of the American Association for Laboratory Animal Science : JAALAS · 2024Article
- A Cross-sectional Survey on Rodent Environmental Health Monitoring Practices: Benchmarking, Associations, and Barriers.Journal of the American Association for Laboratory Animal Science : JAALAS · 2023Article
- Evaluation of In-cage Filter Paper as a Replacement for Sentinel Mice in the Detection of Murine Pathogens.Journal of the American Association for Laboratory Animal Science : JAALAS · 2021Article
- Standardization of an LNA-based TaqMan assay qPCR analysis for Aspiculuris tetraptera DNA in mouse faeces.BMC microbiology · 2020Article
- PCR and RT-PCR in the Diagnosis of Laboratory Animal Infections and in Health Monitoring.Journal of the American Association for Laboratory Animal Science : JAALAS · 2020Article
- Comparing Mouse Health Monitoring Between Soiled-bedding Sentinel and Exhaust Air Dust Surveillance Programs.Journal of the American Association for Laboratory Animal Science : JAALAS · 2020Article
- PCR Testing of IVC Filter Tops as a Method for Detecting Murine Pinworms and Fur Mites.Journal of the American Association for Laboratory Animal Science : JAALAS · 2017Article
- Evaluation of Anthelmintic Resistance and Exhaust Air Dust PCR as a Diagnostic Tool in Mice Enzootically Infected withJournal of the American Association for Laboratory Animal Science : JAALAS · 2017Article
- Interspecies Variation in the Susceptibility of a Wild-Derived Colony of Mice to Pinworms (Journal of the American Association for Laboratory Animal Science : JAALAS · 2017Article
- Evaluation of Traditional and Contemporary Methods for DetectingJournal of the American Association for Laboratory Animal Science : JAALAS · 2017Article
- Guidance Regarding Sample Collection and Refinement of Fecal Flotation Exam for the Isolation of Aspiculuris tetraptera.Journal of the American Association for Laboratory Animal Science : JAALAS · 2016Article
- Effect of Cage-Wash Temperature on the Removal of Infectious Agents from Caging and the Detection of Infectious Agents on the Filters of Animal Bedding-Disposal Cabinets by PCR Analysis.Journal of the American Association for Laboratory Animal Science : JAALAS · 2015Article
- False-positive results after environmental pinworm PCR testing due to Rhabditid nematodes in Corncob bedding.Journal of the American Association for Laboratory Animal Science : JAALAS · 2014Article
- Combined evaluation of commonly used techniques, including PCR, for diagnosis of mouse fur mites.Journal of the American Association for Laboratory Animal Science : JAALAS · 2014Article
- Efficacy of direct detection of pathogens in naturally infected mice by using a high-density PCR array.Journal of the American Association for Laboratory Animal Science : JAALAS · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pinworm detection in laboratory rodents typically is accomplished by using the tape test or various modifications of fecal flotation test to detect eggs. Direct examination of intestinal contents remains the 'gold standard' for pinworm detection, with the limitation of euthanasia of animals. Here, we compare traditional and real-time PCR methodologies during screening for and confirming the presence of Aspiculuris tetraptera. Two sets of pooled fecal samples collected from each of 521 microisolation cages in a mouse facility suspected to be pinworm-positive were tested by PCR and fecal flotation methods. The number of PCR-positive cages was 48 (9.2%) compared with 5 (0.96%) by the fecal flotation method. All of the cages determined to be positive by fecal flotation were positive by PCR. We evaluated 8 positive cages containing 26 mice from the screening group 5 wk later to confirm the initial findings; for 7 of these cages, PCR results from the initial screening were confirmed by fecal centrifugation concentration (FCC) or direct worm detection. Among the 26 mice, 4 were pinworm-positive by FCC, 5 by maceration, and 16 by PCR. All 4 mice positive by FCC were positive by PCR; PCR was positive for 7 of the 9 mice in which pinworms were detected by FCC or maceration. Our study demonstrates that real-time PCR for survival testing of mice for A. tetraptera effectively augments current detection methods for quarantine and routine health monitoring.
Indexed as
Identifiers
22330785PMC3228929W1491103009What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.