ArticleJournal of the American Chemical Society2012
RNA encapsidation by SV40-derived nanoparticles follows a rapid two-state mechanism.
Article in Journal of the American Chemical Society, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
51 citing papers in PubMed, 101 citations in OpenAlex.
- Polyanionic Sugars Drastically Affect Assembly of Human Papilloma Virus Virus-Like Particles.ACS infectious diseases · 2026Article
- Modulating the Curvature of Protein Self-Assembled Spiral Nanotubules.ACS applied materials & interfaces · 2025Article
- Disassembly of Virus-Like Particles and the Stabilizing Role of the Nucleic Acid Cargo.The journal of physical chemistry. B · 2025Article
- Article
- Stimulus-responsive assembly of nonviral nucleocapsids.Nature communications · 2024Article
- Theoretical Studies on Assembly, Physical Stability, and Dynamics of Viruses.Sub-cellular biochemistry · 2024Review
- Effect of ionic strength on the assembly of simian vacuolating virus capsid protein around poly(styrene sulfonate).The European physical journal. E, Soft matter · 2023Article
- Three-Dimensional Investigations of Virus-Associated Structures in the Nuclei with White Spot Syndrome Virus (WSSV) Infection in Red Swamp Crayfish (Animals : an open access journal from MDPI · 2022Article
- 3D-printed SAXS chamber for controlled in situ dialysis and optical characterization.Journal of synchrotron radiation · 2022Article
- Mechanism of Tubulin Oligomers and Single-Ring Disassembly Catastrophe.The journal of physical chemistry letters · 2022Article
- Asymmetrizing an icosahedral virus capsid by hierarchical assembly of subunits with designed asymmetry.Nature communications · 2021Article
- Physics of viral dynamics.Nature reviews. Physics · 2021Review
- Visualizing a viral genome with contrast variation small angle X-ray scattering.The Journal of biological chemistry · 2020Article
- Article
- Rapidly Forming Early Intermediate Structures Dictate the Pathway of Capsid Assembly.Journal of the American Chemical Society · 2020Article
- Assembly and Stability of Simian Virus 40 Polymorphs.ACS nano · 2020Article
- Revealing in real-time a multistep assembly mechanism for SV40 virus-like particles.Science advances · 2020Article
- pH stability and disassembly mechanism of wild-type simian virus 40.Soft matter · 2020Article
- Synchrotron Scattering Methods for Nanomaterials and Soft Matter Research.Materials (Basel, Switzerland) · 2020Review
- Studying viruses using solution X-ray scattering.Biophysical reviews · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Remarkably, uniform virus-like particles self-assemble in a process that appears to follow a rapid kinetic mechanism. The mechanisms by which spherical viruses assemble from hundreds of capsid proteins around nucleic acid, however, are yet unresolved. Using time-resolved small-angle X-ray scattering (TR-SAXS), we have been able to directly visualize SV40 VP1 pentamers encapsidating short RNA molecules (500mers). This assembly process yields T = 1 icosahedral particles comprised of 12 pentamers and one RNA molecule. The reaction is nearly one-third complete within 35 ms, following a two-state kinetic process with no detectable intermediates. Theoretical analysis of kinetics, using a master equation, shows that the assembly process nucleates at the RNA and continues by a cascade of elongation reactions in which one VP1 pentamer is added at a time, with a rate of approximately 10(9) M(-1) s(-1). The reaction is highly robust and faster than the predicted diffusion limit. The emerging molecular mechanism, which appears to be general to viruses that assemble around nucleic acids, implicates long-ranged electrostatic interactions. The model proposes that the growing nucleo-protein complex acts as an electrostatic antenna that attracts other capsid subunits for the encapsidation process.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.