ArticleBiochemistry2012
Myristoylation exerts direct and allosteric effects on Gα conformation and dynamics in solution.
Article in Biochemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 16 citations in OpenAlex.
- Regulators of G protein signaling in cardiovascular function during pregnancy.Physiological genomics · 2018Review
- Exploring the inhibition mechanism of adenylyl cyclase type 5 by n-terminal myristoylated Gαi1.PLoS computational biology · 2017Article
- Article
- A Conserved Hydrophobic Core in Gαi1 Regulates G Protein Activation and Release from Activated Receptor.The Journal of biological chemistry · 2016Article
- Invited review: Activation of G proteins by GTP and the mechanism of Gα-catalyzed GTP hydrolysis.Biopolymers · 2016Review
- GPS-Lipid: a robust tool for the prediction of multiple lipid modification sites.Scientific reports · 2016Article
- A transient interaction between the phosphate binding loop and switch I contributes to the allosteric network between receptor and nucleotide in Gαi1.The Journal of biological chemistry · 2014Article
- Energetic analysis of the rhodopsin-G-protein complex links the α5 helix to GDP release.Nature structural & molecular biology · 2014Article
- Linking receptor activation to changes in Sw I and II of Gα proteins.Journal of structural biology · 2013Article
- Protein sorting, targeting and trafficking in photoreceptor cells.Progress in retinal and eye research · 2013Review
- Conformational flexibility and structural dynamics in GPCR-mediated G protein activation: a perspective.Journal of molecular biology · 2013Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Coupling of heterotrimeric G proteins to activated G protein-coupled receptors results in nucleotide exchange on the Gα subunit, which in turn decreases its affinity for both Gβγ and activated receptors. N-Terminal myristoylation of Gα subunits aids in membrane localization of inactive G proteins. Despite the presence of the covalently attached myristoyl group, Gα proteins are highly soluble after GTP binding. This study investigated factors facilitating the solubility of the activated, myristoylated protein. In doing so, we also identified myristoylation-dependent differences in regions of Gα known to play important roles in interactions with receptors, effectors, and nucleotide binding. Amide hydrogen-deuterium exchange and site-directed fluorescence of activated proteins revealed a solvent-protected amino terminus that was enhanced by myristoylation. Furthermore, fluorescence quenching confirmed that the myristoylated amino terminus is in proximity to the Switch II region in the activated protein. Myristoylation also stabilized the interaction between the guanine ring and the base of the α5 helix that contacts the bound nucleotide. The allosteric effects of myristoylation on protein structure, function, and localization indicate that the myristoylated amino terminus of Gα(i) functions as a myristoyl switch, with implications for myristoylation in the stabilization of nucleotide binding and in the spatial regulation of G protein signaling.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.