ArticleSmall (Weinheim an der Bergstrasse, Germany)2012
Intracellular protein delivery and gene transfection by electroporation using a microneedle electrode array.
Article in Small (Weinheim an der Bergstrasse, Germany), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 75 citations in OpenAlex.
- Recent advances in electric-stimulus assisted microneedle patches for skin disease treatment.Journal of nanobiotechnology · 2025Review
- Iontophoresis and electroporation-assisted microneedles: advancements and therapeutic potentials in transdermal drug delivery.Drug delivery and translational research · 2025Review
- A small-molecule carrier for the intracellular delivery of a membrane-impermeable protein with retained bioactivity.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Tissue Nanotransfection Silicon Chip and Related Electroporation-Based Technologies for In Vivo Tissue Reprogramming.Nanomaterials (Basel, Switzerland) · 2024Review
- Recent Progress of Rational Modified Nanocarriers for Cytosolic Protein Delivery.Pharmaceutics · 2023Review
- Microneedle-Based Glucose Sensor Platform: FromBiosensors · 2022Review
- In-Plane Si Microneedles: Fabrication, Characterization, Modeling and Applications.Micromachines · 2022Review
- Design and Evaluation of In-Plane Silicon Microneedles Fabricated with Post-CMOS Compatible Processes.Sensors and actuators. A, Physical · 2022Article
- Microneedle systems for delivering nucleic acid drugs.Journal of pharmaceutical investigation · 2022Review
- An ultra-low-cost electroporator with microneedle electrodes (ePatch) for SARS-CoV-2 vaccination.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- A Simple and Quick Method for Loading Proteins in Extracellular Vesicles.Pharmaceuticals (Basel, Switzerland) · 2021Article
- Microneedles Drug Delivery Systems for Treatment of Cancer: A Recent Update.Pharmaceutics · 2020Review
- Engineering Antiviral Vaccines.ACS nano · 2020Review
- Cytosolic Delivery of Functional ProteinsACS applied materials & interfaces · 2020Article
- Protein delivery into cells using inorganic nanoparticle-protein supramolecular assemblies.Chemical Society reviews · 2018Review
- Efficient delivery of nucleic acid molecules into skin by combined use of microneedle roller and flexible interdigitated electroporation array.Theranostics · 2018Article
- Advanced Gene Manipulation Methods for Stem Cell Theranostics.Theranostics · 2017Review
- A novel electroporation system for efficient molecular delivery into Chlamydomonas reinhardtii with a 3-dimensional microelectrode.Scientific reports · 2015Article
- Inducing Stem Cell Myogenesis Using NanoScript.ACS nano · 2015Article
- Microfluidic squeezing for intracellular antigen loading in polyclonal B-cells as cellular vaccines.Scientific reports · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
The impact of many biopharmaceuticals, including protein- and gene-based therapies, has been limited by the need for better methods of delivery into cells within tissues. Here, intracellular delivery of molecules and transfection with plasmid DNA by electroporation is presented using a novel microneedle electrode array designed for the targeted treatment of skin and other tissue surfaces. The microneedle array is molded out of polylactic acid. Electrodes and circuitry required for electroporation are applied to the microneedle array surface by a new metal-transfer micromolding method. The microneedle array maintains mechanical integrity after insertion into pig cadaver skin and is able to electroporate human prostate cancer cells in vitro. Quantitative measurements show that increasing electroporation pulse voltage increases uptake efficiency of calcein and bovine serum albumin, whereas increasing pulse length has lesser effects over the range studied. Uptake of molecules by up to 50% of cells and transfection of 12% of cells with a gene for green fluorescent protein is demonstrated at high cell viability. It is concluded that the microneedle electrode array is able to electroporate cells, resulting in intracellular uptake of molecules, and has potential applications to improve intracellular delivery of proteins, DNA, and other biopharmaceuticals.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.